Human M-Cadherin (extracellular domain) antibody

Antigen: Human M-Cadherin

Epitope: extracellular domain

Clonality: Monoclonal (21G4)

Host: Mouse

Reactivity: Human

Application: ELISA, Immunofluorescence (IF), Western Blotting (WB)

Catalog no.: ABIN234424

Additional Information

Alternative name: Human M-Cadherin (extracellular domain)

Immunogen: Recombinant extracellular domain of human M-Cadherin

Format: Purified, Lyophilized

Isotype: IgG1  (Matching secondary antibodies)

Clone: 21G4

Description: MAb to M-Cadherin Monoclonal Antibody to Human M-Cadherin (extracellular domain)

Specificity: Cadherins are a family of transmembrane glycoproteins that play a key role in Calcium dependent cell-cell adhesion. Several members of the cadherin family have been identified so far, including E- (epithelial), P- (placental), N- (neuronal) and M- (muscle) cadherin. M-Cadherin is expressed in myoblasts and might be involved in skeletal muscle cell differentiation. Does not cross-react with mouse.

Application Details

Application Notes: Immunoblotting: 1ug/ml for AP/BCIP/NBT (MTT) detection, 0.5ug/ml for HRP/ECL detection. Western blot Analysis Whole cell lysates (lane 1: COS-7, lane 2: human M-cadherin overexpressed in COS-7 cells) were applied to SDS-PAGE and transferred to PVDF membrane. The blot was probed with 0.5?g/ml H67214M for 1 hour at RT and developed by ECL (exp. Time: 30 sec). ELISA: 0.05ug/ml Immunofluorescence: 5ug/ml Each laboratory should determine an optimum working titer for use in its particular application. Other applications have not been tested but use in such assays should not necessarily be excluded.

Concentration: 100ug/ml (prior to lyophilization)

Purification: Thiophilic adsorption and size exclusion chromatography. Source: Cell culture

Buffer: Lyophilized from 2X PBS, PEG and Sucrose. Preservative: 0.09% Sodium azide

Storage: Store lyophilized product at -20°C. After reconstitution, aliquot and store at -80°C. Thawed aliquots may be stored at 2-8°C for up to 3 months. Avoid multiple freeze/thaw cycles.Reconstitute with 1ml water for 15 minutes at room temperature.

Restrictions: For Research Use only

Images

Western blot Analysis: Whole cell lysates (lane 1: COS-7, lane 2: human M-cadherin overexpressed in COS-7 cells) were applied to SDS-PAGE and transferred to PVDF membrane. The blot was probed with 0.5µg/ml ABIN234424 for 1 hour at RT and developed by ECL (exp. Time: 30 sec).