beta-Catenin (CTNNB1) antibody

Antigen: beta-Catenin (CTNNB1)

Synonyms: CTNNB, FLJ25606, FLJ37923, DKFZp686D02253, Mesc, Catnb, ctnnb, id:ibd2058, wu:fb73e10, wu:fi81c06, wu:fk25h01, ARM, Beta-cat, Beta-catenin, EG:86E4.6, beta-cat-arm, l(1)2Bv, l(1)G0192, l(1)G0234, l(1)G0410, l(1)arm, t12687 ALR Dm, DmelCG11579, CG11579, beta-catenin, ctnnb1, MGC79992, MGC132060, Cibeta-catenin, CTNNB1, MGC52601, LOC576711, Ctnnb1, MGC139922

Clonality: Monoclonal (BDI480)

Host: Mouse

Application: ELISA, Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunocytochemistry (ICC)

Catalog no.: ABIN234473

Additional Information

Alternative name: Catenin, beta

Immunogen: Synthetic peptide Format: Purified, Lyophilized Reconstitute with 1ml water for 15 minutes at room temperature

Format: Purified, Lyophilized

Isotype: IgG1  (Matching secondary antibodies)

Clone: BDI480

Description: Cell lysate of 2 x 106 LiCl treated SW480 cells Cell culture

Specificity: Specifically interacts with beta-catenin. The alpha-, beta- and gamma-catenins are cytoplasmic proteins mediating the interaction of Ca2+ -dependent transmembrane adhesion molecules (cadherins) with the cytoskeletal network. The direct interaction of beta-catenin with the cytoplasmic domain of cadherins plays a crucial role for cell-cell adhesion and signal transmission between neighboring cells. Recent studies indicate that beta-catenin may also play a role in tumorigenesis since it forms complexes with the tumor suppressor gene product APC. Beta-catenin directly interacts and constitutively activates transcription factors of the TCF/LEF gene family. Thus it is proposed that beta-catenin plays a dual role not only in the maintenance and regulation of cell-cell interactions but also in the regulation of gene activity. Cross reacts with human, mouse and dog.

Synonyms: CTNNB, FLJ25606, FLJ37923, DKFZp686D02253, Mesc, Catnb, ctnnb, id:ibd2058, wu:fb73e10, wu:fi81c06, wu:fk25h01, ARM, Beta-cat, Beta-catenin, EG:86E4.6, beta-cat-arm, l(1)2Bv, l(1)G0192, l(1)G0234, l(1)G0410, l(1)arm, t12687 ALR Dm, DmelCG11579, CG11579, beta-catenin, ctnnb1, MGC79992, MGC132060, Cibeta-catenin, CTNNB1, MGC52601, LOC576711, Ctnnb1, MGC139922

Application Details

Application Notes: Western blot: 0.5ug/ml for HRP/ECL detection. Recommended blocking buffer CPPT: 0.5% (w/v) casein, 1% (w/v) PEG 4000, 1% (w/v) Polyvinylpyrrolidone (PVP), 0.1% Tween 20, 10mM Tris/HCl, pH 7.4, 150mM Sodium Chloride. DO NOT USE MILK FOR BLOCKING! Detection of endogenous ?-Catenin Whole cell lysates of serum starved tumor cells (20,000 cells per lane) were applied to SDS-PAGE and transferred to a PVDF membrane. The blot was probed with 0.5?g/ml K01291M for 1 hour at RT and developed by ECL (exp. time: 30 sec). Lane 1: A431 Lane 5: MCF7 Lane 2: SW480 Lane 6: MDA-MB231 Lane 3: SW620 Lane 7: T47D Lane 4: HT29 Immunocytochemistry: 1?10ug/ml ELISA: 0.05ug/ml Each laboratory should determine an optimum working titer for use in its particular application. Other applications have not been tested but use in such assays should not necessarily be excluded. Storage: Store lyophilized product at -20?C. After reconstitution, aliquot and store at -80?C. Thawed aliquots may be stored at 2?8?C for up to 3 months. Warning:

Concentration: 100ug/ml (prior to lyophilization)

Purification: Thiophilic adsorption and size exclusion chromatography. Source: Cell culture

Buffer: Lyophilized from 2X PBS, PEG and Sucrose. Preservative: 0.1% Sodium azide

Storage: Store lyophilized product at -20°C. After reconstitution, aliquot and store at -80°C. Thawed aliquots may be stored at 2-8°C for up to 3 months. Warning:Reconstitute with 1ml water for 15 minutes at room temperature Purification:

Restrictions: For Research Use only

Images

Detection of endogenous beta-Catenin. Whole cell lysates of serum starved tumor cells (20,000 cells per lane) were applied to SDS-PAGE and transferred to a PVDF membrane. The blot was probed with 0.5ug/ml ABIN234473 for 1 hour at RT and developed by ECL (exp. time: 30 sec). Lane 1: A431. Lane 2: SW480. Lane 3: SW620. Lane 4: HT29. Lane 5: MCF7. Lane 6: MDA-MB231. Lane 7: T47D