Killer Cell Immunoglobulin-Like Receptor, Two Domains, Long Cytoplasmic Tail, 2 (KIR2DL2) antibody

Antigen: Killer Cell Immunoglobulin-Like Receptor, Two Domains, Long Cytoplasmic Tail, 2 (KIR2DL2)

Synonyms: NKAT6, p58.2, CD158b, CD158B1

Clonality: Monoclonal (GL183)

Host: Mouse

Reactivity: Human

Conjugate: Un-conjugated

Application: Immunoprecipitation (IP), Flow Cytometry (FACS)

Catalog no.: ABIN234532

Additional Information

Alternative name: CD158b (p58.2)

Immunogen: NK cell clone E57 (CD2+, CD3-, CD7+, CD16+, CD56+)

Format: Lyophilized

Isotype: IgG1

Clone: GL183

Description: MAb to Human CD158b (p58.2) Monoclonal Antibody to Human CD158b (p58.2)

Specificity: GL183 monoclonal antibody immunoprecipitates the p58.2 molecule, a 58kDa type I transmembrane protein (1-3). It can also react with a lighter (p50.2, 50kDa) molecule, highly homologous to p58.2 in the extracellular area, but exhibiting a shorter cytoplasmic tail.(4,5) These p58.2 and 50.2 molecules are characterized by two immunoglobulin-like extracellular domains. They are expressed by human NK cell subsets and by human T lymphocytes subsets, the latest being, in most instances, CD8+CD4-TCR alpha beta+.(6,7) p58.2 and p50.2 are members of the newly described natural killer cell receptor (NK-R) family (for reviews, see 8,9). The GL183 antibody can restore in vitro the lysis by human NK clones of otherwise lysis-protected targets carrying the Cw3 or related alleles. GL183 antibody has been assigned to the CD158b cluster of differentiation of the VIth International Workshop on Human Leucocyte Differentiation Antigens (HLDA) in Kobe, 1996.

Application Details

Application Notes: Flow cytometry: 2ug / 5 x 105 cells (or 100ul whole blood), per test. Studies on the recognition mechanisms and function of NK cells and CTLs. Analysis of the NK cell repertoire. Immunoprecipitation of p50.2 and p58.2 molecules. Each laboratory should determine an optimum working titer for use in its particular application. Other applications have not been tested but use in such assays should not necessarily be excluded. FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES. Catalog P91846M Page 2 of 2

Concentration: 0.2mg/ml (prior to lyophilization)

Purification: Protein A chromatography. Source: Ascites

Buffer: Lyophilized from PBS containing 1mg/ml BSA.

Storage: Store lyophilized product at 2-8°C. After reconstitution, aliquot and store at -20°C. Avoid multiple freeze/thaw cycles. The addition of 0.1% (w/v) sodium azide is recommended for storage of the reconstituted liquid for up to one month at 2-8°C.Reconstitute with 1ml distilled water.

Restrictions: For Research Use only

Publications

Moretta, Tambussi, Bottino et al.: "A novel surface antigen expressed by a subset of human CD3- CD16+ natural killer cells. Role in cell activation and regulation of cytolytic function." in: The Journal of experimental medicine, Vol. 171, Issue 3, pp. 695-714, 1990 (PubMed).

Moretta, Bottino, Pende et al.: "Identification of four subsets of human CD3-CD16+ natural killer (NK) cells by the expression of clonally distributed functional surface molecules: correlation between subset assignment of NK clones and ability to mediate specific alloantigen recognition." in: The Journal of experimental medicine, Vol. 172, Issue 6, pp. 1589-98, 1991 (PubMed).

Moretta, Sivori, Vitale et al.: "Existence of both inhibitory (p58) and activatory (p50) receptors for HLA-C molecules in human natural killer cells." in: The Journal of experimental medicine, Vol. 182, Issue 3, pp. 875-84, 1995 (PubMed).

Wagtmann, Biassoni, Cantoni et al.: "Molecular clones of the p58 NK cell receptor reveal immunoglobulin-related molecules with diversity in both the extra- and intracellular domains." in: Immunity, Vol. 2, Issue 5, pp. 439-49, 1995 (PubMed).

Ferrini, Cambiaggi, Meazza et al.: "T cell clones expressing the natural killer cell-related p58 receptor molecule display heterogeneity in phenotypic properties and p58 function." in: European journal of immunology, Vol. 24, Issue 10, pp. 2294-8, 1994 (PubMed).

Biassoni, Cantoni, Falco et al.: "The human leukocyte antigen (HLA)-C-specific "activatory" or "inhibitory" natural killer cell receptors display highly homologous extracellular domains but differ in their transmembrane and intracytoplasmic portions." in: The Journal of experimental medicine, Vol. 183, Issue 2, pp. 645-50, 1996 (PubMed).

Mingari, Schiavetti, Ponte et al.: "Human CD8+ T lymphocyte subsets that express HLA class I-specific inhibitory receptors represent oligoclonally or monoclonally expanded cell populations." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 93, Issue 22, pp. 12433-8, 1996 (PubMed).

Lanier: "Natural killer cell receptors and MHC class I interactions." in: Current opinion in immunology, Vol. 9, Issue 1, pp. 126-31, 1997 (PubMed).