CD19, gp95 antibody

Antigen: CD19, gp95

Clonality: Monoclonal (J4-119)

Host: Mouse

Reactivity: Human

Application: Flow Cytometry (FACS)

Catalog no.: ABIN234548

Additional Information

Immunogen: SKLY 18 lymphoma cells

Format: Lyophilized

Isotype: IgG1

Clone: J4-119

Description: MAb to CD19 Monoclonal Antibody to CD19, gp95

Specificity: CD19 antigen is a single chain, type I integral membrane glycoprotein with a molecular weight of 95kDa. J4.119 cross-reacts with B cells from Macaca fascicularis, Macaca mulatta and Papio cynocephalus . It reacts weakly with B cells from Rhesus monkey. The J4.119 MAb was assigned to the CD19 cluster for differentiation at the 4th International Workshop on Human Leucocyte Differentiation Antigens (HDLA) in Vienna, Austria (1989).

Application Details

Application Notes: Study of CD19 expressing lymphocytes. Flow cytometry: 2?g reconstituted antibody per 5x105 cells/test or 100ul blood sample. Each laboratory should determine an optimum working titer for use in its particular application. Other applications have not been tested but use in such assays should not necessarily be excluded.

Concentration: 0.2mg/ml (prior to lyophilization)

Purification: Cation exchange chromatography. Source: Ascites

Buffer: Lyophilized from PBS containing 1mg/ml BSA.

Storage: Store lyophilized product at 2-8°C. After reconstitution, aliquot and store at -20°C. Avoid multiple freeze/thaw cycles. The addition of 0.1% sodium azide is recommended for storage up to one month when stored at 2-8°C.Reconstitute with 1ml distilled water.

Restrictions: For Research Use only

Publications

Freedman, Nadler: "Cell surface markers in hematologic malignancies." in: Seminars in oncology, Vol. 14, Issue 2, pp. 193-212, 1987 (PubMed).

Campana, Janossy: "Leukemia diagnosis and testing of complement-fixing antibodies for bone marrow purging in acute lymphoid leukemia." in: Blood, Vol. 68, Issue 6, pp. 1264-71, 1987 (PubMed).

Nadler, Anderson, Marti et al.: "B4, a human B lymphocyte-associated antigen expressed on normal, mitogen-activated, and malignant B lymphocytes." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 131, Issue 1, pp. 244-50, 1983 (PubMed).

Melsom, Funderud, Lie et al.: "The great majority of childhood lymphoblastic leukaemias are identified by monoclonal antibodies as neoplasias of the B-cell progenitor compartment." in: Scandinavian journal of haematology, Vol. 33, Issue 1, pp. 27-34, 1984 (PubMed).