CD19, Gp95 antibody

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Clonality (Clone)
Monoclonal ()
Flow Cytometry (FACS)
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Immunogen SKLY 18 lymphoma cells
Clone J4-119
Isotype IgG1
Specificity CD19 antigen is a single chain, type I integral membrane glycoprotein with a molecular weight of 95 kDa. J4.119 cross-reacts with B cells from Macaca fascicularis, Macaca mulatta and Papio cynocephalus . It reacts weakly with B cells from Rhesus monkey. The J4.119 MAb was assigned to the CD19 cluster for differentiation at the 4th International Workshop on Human Leucocyte Differentiation Antigens (HDLA) in Vienna, Austria (1989).
Characteristics MAb to CD19, Monoclonal antibody to CD19, gp95
Purification CatIon Exchange Chromatography. Ascites
Application Notes Study of CD19 expressing lymphocytes. Flow cytometry: 2-g reconstituted antibody per 5x105 cells/test or 100 µL blood sample. Each laboratory should determine an optimum working titer for use in its particular application. Other applications have not been tested but use in such assays should not necessarily be excluded.
Restrictions For Research Use only
Format Lyophilized
Reconstitution Reconstitute with 1 mL distilled water.
Buffer Lyophilized from PBS containing 1 mg/mL BSA.
Preservative Without preservative
Handling Advice Avoid multiple freeze/thaw cycles. The addition of 0.1 % sodium azide is recommended for storage up to one month when stored at 2-8 °C.
Centrifuge product if not completely clear after standing at room temperature.
Prepare working dilution only prior to immediate use.
Storage 4 °C/-20 °C
Storage Comment Store lyophilized product at 2-8 °C. After reconstitution, aliquot and store at -28 °C.
Background publications Campana, Janossy: "Leukemia diagnosis and testing of complement-fixing antibodies for bone marrow purging in acute lymphoid leukemia." in: Blood, Vol. 68, Issue 6, pp. 1264-71, 1987 (PubMed).

Freedman, Nadler: "Cell surface markers in hematologic malignancies." in: Seminars in oncology, Vol. 14, Issue 2, pp. 193-212, 1987 (PubMed).

Melsom, Funderud, Lie, Godal: "The great majority of childhood lymphoblastic leukaemias are identified by monoclonal antibodies as neoplasias of the B-cell progenitor compartment." in: Scandinavian journal of haematology, Vol. 33, Issue 1, pp. 27-34, 1984 (PubMed).

Nadler, Anderson, Marti, Bates, Park, Daley, Schlossman: "B4, a human B lymphocyte-associated antigen expressed on normal, mitogen-activated, and malignant B lymphocytes." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 131, Issue 1, pp. 244-50, 1983 (PubMed).