CD19 Pan B-Cell antibody (FITC)

Antigen: CD19 Pan B-Cell

Clonality: Monoclonal (J4-119)

Host: Mouse

Reactivity: Human

Conjugate: FITC

Application: Flow Cytometry (FACS)

Catalog no.: ABIN234549

Additional Information

Immunogen: SKLY 18 lymphoma cells

Format: Liquid

Isotype: IgG1

Clone: J4-119

Description: MAb to CD19 Monoclonal Antibody to CD19 Pan B-Cell Fluorescein conjugated

Specificity: The molecular weight of the recognized antigen is 95kDa

Application Details

Application Notes: Characterization of B cells Study of activation and proliferation mechanisms of B cells Flow cytometry: 20ul/5x105 cells/test or 100ul whole blood. Each laboratory should determine an optimum working titer for use in its particular application. Other applications have not been tested but use in such assays should not necessarily be excluded.

Concentration: Not determined.

Buffer: PBS, pH 7.2 containing 2mg/ml BSA. Preservative: 0.1% Sodium azide

Storage: Store at 2-8°C. DO NOT FREEZE!

Restrictions: For Research Use only

Publications

Uckun: "Regulation of human B-cell ontogeny." in: Blood, Vol. 76, Issue 10, pp. 1908-23, 1990 (PubMed).

Loken, Shah, Dattilio et al.: "Flow cytometric analysis of human bone marrow. II. Normal B lymphocyte development." in: Blood, Vol. 70, Issue 5, pp. 1316-24, 1987 (PubMed).

Maserati, Pesando, Filice et al.: "[Prospects of antibiotic prophylaxis in cesarean section]" in: Giornale italiano di chemioterapia, Vol. 29, Issue 1, pp. 45-7, 1983 (PubMed).

Doody, Dempsey, Fearon: "Activation of B lymphocytes: integrating signals from CD19, CD22 and Fc gamma RIIb1." in: Current opinion in immunology, Vol. 8, Issue 3, pp. 378-82, 1997 (PubMed).

Carter, Doody, Bolen et al.: "Membrane IgM-induced tyrosine phosphorylation of CD19 requires a CD19 domain that mediates association with components of the B cell antigen receptor complex." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 158, Issue 7, pp. 3062-9, 1997 (PubMed).

Kurosaki: "Molecular mechanisms in B cell antigen receptor signaling." in: Current opinion in immunology, Vol. 9, Issue 3, pp. 309-18, 1997 (PubMed).