CD2 (LFA-2) antibody

Antigen: CD2 (LFA-2)

Clonality: Monoclonal (6F10-3)

Host: Mouse

Reactivity: Human

Application: Flow Cytometry (FACS)

Catalog no.: ABIN234556

Additional Information

Alternative name: CD2 gp 50kDa

Immunogen: Thymocytes

Format: Lyophilized

Isotype: IgG1

Clone: 6F10-3

Description: MAb to CD2 Monoclonal Antibody to CD2 gp 50kDa

Specificity: CD2 is a group of antibodies which recognizes the T cell receptor for sheep red blood cells. This receptor is present on 80% of normal human peripheral blood lymphocytes, 95% of thymocytes, 100% of T lymphocytes and on a part of Natural Killer cells. Several epitopes can be distinguished on the target molecule. The antibody 6F10.3 reacts with the T11-2 epitope. The clone 39C1.5 (Catalog P42395M) reacts with the T11-1 epitope. The combination of the monoclonal antibodies is strongly mitogenic in the presence of TPA.

Application Details

Application Notes: Study of T cell functions Fluorescence microscopy or flow cytometry: 2 microgram/5x105 cells/test Immunohistochemistry: working dilution 1:25 to 1:50 This antibody is only suitable on cryostat sections or cell smears Each laboratory should determine an optimum working titer for use in its particular application. Other applications have not been tested but use in such assays should not necessarily be excluded.? FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES. Catalog P42180M Page 2 of 2

Concentration: Not applicable

Purification: Protein A Sepharose chromatography. Source: Ascites

Buffer: Lyophilized from PBS containing 1mg/ml BSA.

Storage: Store lyophilized product at 2-8°C. The addition of 0.1% (w/v) sodium azide is recommended for storage up to one month at 2-8°C or several months at -20°C after being aliquoted.Reconstitute with 1ml distilled water.

Restrictions: For Research Use only

Publications

Breitmeyer, Daley, Levine et al.: "The T11 (CD2) molecule is functionally linked to the T3/Ti T cell receptor in the majority of T cells." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 139, Issue 9, pp. 2899-905, 1987 (PubMed).

Huet, Boumsell, Raynal et al.: "Role in T-cell activation for HLA class I molecules from accessory cells: further distinction between activation signals delivered to T cells via CD2 and CD3 molecules." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 84, Issue 20, pp. 7222-6, 1987 (PubMed).

Meuer, Hussey, Fabbi et al.: "An alternative pathway of T-cell activation: a functional role for the 50 kd T11 sheep erythrocyte receptor protein." in: Cell, Vol. 36, Issue 4, pp. 897-906, 1984 (PubMed).