CD28 (CD28) antibody

Antigen: CD28 (CD28)

Synonyms: Tp44, MGC138290

Clonality: Monoclonal (CD28-2)

Host: Mouse

Reactivity: Human

Application: Flow Cytometry (FACS)

Catalog no.: ABIN234576

Additional Information

Alternative name: CD28

Immunogen: Transfected murine cell line

Format: Purified, Liquid

Isotype: IgG1  (Matching secondary antibodies)

Clone: CD28-2

Description: MAb to CD28 Monoclonal Antibody to CD28

Specificity: CD28 is a T-cell surface homodimeric 44kDa molecule that belongs to the Ig superfamily (1). CD28 is involved in cell adhesion T-B through the binding to its ligand B7/BB1, which is also the ligand for CTLA-4. CD28-B7 interaction is an important cosignal that allow T-cell proliferation and lymphokine production. CD28 is associated to a PI3 kinase activity (2). The majority of CD4+ T-cells and 50% of CD8+ T-cells express CD28 (3). The CD28.2 monoclonal antibody induces T-cell proliferation in costimulation with CD2 monoclonal antibodies (2). It also inhibits the CD4+ proliferation in the allogeneic T-cell response (2). This antibody has been studied at the Vth International Workshop on Human Leucocyte Differentiation Antigens in Boston (1993).

Application Details

Application Notes: Flow cytometry: 20ul/5x105 cells/test Signal transduction T-lymphocyte activation studies. T and B cell adhesion studies. Each laboratory should determine an optimum working titer for use in its particular application. Other applications have not been tested but use in such assays should not necessarily be excluded.

Concentration: 25ug/ml

Purification: Anion exchange chromatography. Source: Ascites

Buffer: PBS containing 2mg/ml BSA. Preservative: 0.1% Sodium azide

Storage: Store at 2-8°C.

Research Area: Immunology, Adaptive Immunity, Hematopoietic Progenitors, CD Antigens, Surface Receptors of Immune Cells, Apoptosis/Necrosis

Restrictions: For Research Use only

Publications

Publications: Nunès, Klasen, Ragueneau et al.: "CD28 mAbs with distinct binding properties differ in their ability to induce T cell activation: analysis of early and late activation events." in: International immunology, Vol. 5, Issue 3, pp. 311-5, 1993 (PubMed).