CD3e Molecule, epsilon (CD3-TCR Complex) (CD3E) antibody

Antigen: CD3e Molecule, epsilon (CD3-TCR Complex) (CD3E)

Synonyms: T3E, TCRE, FLJ18683, CD3E, cd3e, CD3, t3e, tcre

Clonality: Monoclonal (500-A2)

Host: Syrian Hamster

Reactivity: Mouse (Murine)

Application: Immunofluorescence (IF), Flow Cytometry (FACS)

Catalog no.: ABIN234587

Additional Information

Alternative name: Mouse CD3 epsilon

Immunogen: C6VL-BS cell lysate

Format: Purified, Liquid

Isotype: IgG  (Matching secondary antibodies)

Clone: 500-A2

Description: MAb to Mouse CD3 Epsilon Monoclonal Antibody to Mouse CD3 epsilon

Specificity: Anti-Mouse CD3 epsilon recognizes the (Mr 25kD) epsilon chain of the CD3 T-cell receptor complex expressed on mouse T-cells. The CD3 epsilon is present on thymocytes, CD8+ and CD4+ positive cells expressed low level of CD3 epsilon while cells expressing high levels of CD3 epsilon were either CD8+ or CD4+.

Application Details

Application Notes: Anti-CD3 epsilon antibody can be used for flow cytometric analysis, immunofluorescence and immunohistochemical staining of acetone fixed fresh frozen tissue sections. We recommend using 1ug to stain 1.0 x 106 cells in flow cytometric applications. Each laboratory should determine an optimum working titer for use in its particular application. Other applications have not been tested but use in such assays should not necessarily be excluded.

Concentration: 0.5mg/ml (OD280nm)

Purification: Protein G chromatography. Source: Tissue culture

Buffer: 0.01M PBS, pH 7.2 containing 1% BSA. Preservative: 0.09% Sodium Azide

Storage: Short-term (up to 6 months) store at 2-8°C. Long-term, aliquot and store at -20°C. Avoid multiple freeze/thaw cycles.

Restrictions: For Research Use only