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CD3 (CD3E) antibody

Antigen

CD3 (CD3E)
Synonyms T3E, TCRE, FLJ18683
Clonality Monoclonal (UCHT1)
Host
Alternatives

Mouse
Reactivity
Alternatives

Human
Conjugate
Alternatives Un-conjugated
Application
Alternatives Flow Cytometry (FACS)
6 references available
Catalog no. ABIN234588
Quantity 100 Tests  (12.5ug/ml (sufficient for 100 Tests...)  (Variants)
Price 349.80 $   Plus shipping costs $45.00
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Additional Information
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Alternative name CD3
Immunogen Peripheral blood lymphocytes
Format Liquid
Isotype IgG1
Clone UCHT1
Description MAb to CD3 Monoclonal Antibody to CD3
Specificity Reacts with the epsilon chain of the CD3 complex.(5) CD3 is associated with the T cell receptor (TcR) on T lymphocytes and is involved in the recognition of peptides bound to the major histocompatibility complex (MHC) during the immune response.(2) The CD3 antigen is expressed by mature T lymphocytes and by a subset of thymocytes.(1, 3)

Application Details
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Application Notes Studies of CD3 expressing lymphocytes and intracellular studies of the CD3 epsilon chain. Flow cytometry: 20ul/5x105 cells or 100ul of whole blood. Each laboratory should determine an optimum working titer for use in its particular application. Other applications have not been tested but use in such assays should not necessarily be excluded.
Concentration 12.5ug/ml (sufficient for 100 Tests)
Purification Purified
Buffer PBS containing 2mg/ml BSA. Preservative: 0.1% Sodium Azide
Storage Store at 2-8°C. DO NOT FREEZE!
Research Area Immunology, Adaptive Immunity, Hematopoietic Progenitors, Hematopoietic Stem Cells, CD Antigens, Surface Receptors of Immune Cells
Restrictions For Research Use only

Publications
Application Details Alternatives Variants back to top

le Gouvello, Manceau, Sobel: "Serine 16 of stathmin as a cytosolic target for Ca2+/calmodulin-dependent kinase II after CD2 triggering of human T lymphocytes." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 161, Issue 3, pp. 1113-22, 1998 (PubMed).

Torres, Alcover, Zapata et al.: "TCR dynamics in human mature T lymphocytes lacking CD3 gamma." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 170, Issue 12, pp. 5947-55, 2003 (PubMed).

Arnett, Harrison, Wiley: "Crystal structure of a human CD3-epsilon/delta dimer in complex with a UCHT1 single-chain antibody fragment." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 101, Issue 46, pp. 16268-73, 2004 (PubMed).

Demedts, Brusselle, Vermaelen et al.: "Identification and characterization of human pulmonary dendritic cells." in: American journal of respiratory cell and molecular biology, Vol. 32, Issue 3, pp. 177-84, 2005 (PubMed).

Lin, Liu, Chen et al.: "CD94 1A transcripts characterize lymphoblastic lymphoma/leukemia of immature natural killer cell origin with distinct clinical features." in: Blood, Vol. 106, Issue 10, pp. 3567-74, 2005 (PubMed).

Rieux-Laucat, Hivroz, Lim et al.: "Inherited and somatic CD3zeta mutations in a patient with T-cell deficiency." in: The New England journal of medicine, Vol. 354, Issue 18, pp. 1913-21, 2006 (PubMed).

Alternatives
Application Details Publications Variants back to top

Alternatives for antigen "CD3 (CD3E)", type "Antibodies"
Hosts Mouse (318), Rabbit (72), Rat (55), Hamster (39)
Reactivities Human (323), Mouse (Murine) (109), Rat (Rattus) (50), Dog (Canine) (20), Primate (15), Monkey (4), Cat (Feline) (1), Cow (Bovine) (1), Horse (Equine) (1), Rhesus Monkey (1)
Applications Flow Cytometry (FACS) (337), Immunoprecipitation (IP) (85), Functional Studies (Func) (76), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (62), Western Blotting (WB) (56), Immunohistochemistry (IHC) (53), Immunofluorescence (IF) (38), Immunohistochemistry (Fixed) (IHC (fx)) (33), Immunohistochemistry (Frozen Sections) (IHC (fro)) (33), ELISA (29), Immunocytochemistry (ICC) (7), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (7), Immunostaining (ISt) (4), Activation (Act) (3), Blocking Antibody (Inhibition) (3), Blocking Antibody (Inhibition) (2), Cytotoxicity Test (CyTox) (1), Dot Blot (Dot) (1), Fluorescence Microscopy (FM) (1), Immunoelectron Microscopy (IEM) (1)
Conjugates FITC (80), Biotin (48), PE (39), APC (27), RPE (7), Cy5 (6), PerCP (5), Alexa Fluor 488 (4), Cy5,PE (4), PE,Cy5 (3), PE,Cy7 (3), PE-Dyomics 647 (3), RPE,Cy5 (3), SPRD (3), APC,Cy7 (2), Alexa Fluor 647 (2), Cy5.5 (2), Cy7,PE (2), Dyomics 647 (2), PE,Cy5.5 (2), PE-Dyomics 590 (2), Alexa Fluor 350 (1), Alexa Fluor 555 (1), CF405M (1), Cy3 (1), Cy7 (1), Gold (1), HRP (1), Orange Cytognos 515 (1), PE,Cy3 (1), PE,DY647 (1), PE,Dyomics 647 (1), PE-Cy5 (1), PerCP,Cy5.5 (1), PerCP-Cy5.5 (1), RPE,Cy7 (1)
Epitopes Chain delta (8), AA 156-168 (7), Cytoplasmic Domain (7), Chain epsilon (6), Activation Site (4), AA 196-206 (1), C-Term (1), Chain gamma,N-Term (1), Internal Region (1)