CD3 (CD3E) antibody

Antigen: CD3 (CD3E)

Synonyms: T3E, TCRE, FLJ18683

Clonality: Monoclonal (UCHT1)

Host: Mouse

Reactivity: Human

Application: Flow Cytometry (FACS)

Catalog no.: ABIN234588

Additional Information

Alternative name: CD3

Immunogen: Peripheral blood lymphocytes

Format: Purified, Liquid

Isotype: IgG1  (Matching secondary antibodies)

Clone: UCHT1

Description: MAb to CD3 Monoclonal Antibody to CD3

Specificity: Reacts with the epsilon chain of the CD3 complex.(5) CD3 is associated with the T cell receptor (TcR) on T lymphocytes and is involved in the recognition of peptides bound to the major histocompatibility complex (MHC) during the immune response.(2) The CD3 antigen is expressed by mature T lymphocytes and by a subset of thymocytes.(1, 3)

Application Details

Application Notes: Studies of CD3 expressing lymphocytes and intracellular studies of the CD3 epsilon chain. Flow cytometry: 20ul/5x105 cells or 100ul of whole blood. Each laboratory should determine an optimum working titer for use in its particular application. Other applications have not been tested but use in such assays should not necessarily be excluded.

Concentration: 12.5ug/ml (sufficient for 100 Tests)

Buffer: PBS containing 2mg/ml BSA. Preservative: 0.1% Sodium Azide

Storage: Store at 2-8°C. DO NOT FREEZE!

Research Area: Immunology, Adaptive Immunity, Hematopoietic Progenitors, Hematopoietic Stem Cells, CD Antigens, Surface Receptors of Immune Cells

Restrictions: For Research Use only

Publications

Publications: le Gouvello, Manceau, Sobel: "Serine 16 of stathmin as a cytosolic target for Ca2+/calmodulin-dependent kinase II after CD2 triggering of human T lymphocytes." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 161, Issue 3, pp. 1113-22, 1998 (PubMed).

Torres, Alcover, Zapata et al.: "TCR dynamics in human mature T lymphocytes lacking CD3 gamma." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 170, Issue 12, pp. 5947-55, 2003 (PubMed).

Arnett, Harrison, Wiley: "Crystal structure of a human CD3-epsilon/delta dimer in complex with a UCHT1 single-chain antibody fragment." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 101, Issue 46, pp. 16268-73, 2004 (PubMed).

Demedts, Brusselle, Vermaelen et al.: "Identification and characterization of human pulmonary dendritic cells." in: American journal of respiratory cell and molecular biology, Vol. 32, Issue 3, pp. 177-84, 2005 (PubMed).

Lin, Liu, Chen et al.: "CD94 1A transcripts characterize lymphoblastic lymphoma/leukemia of immature natural killer cell origin with distinct clinical features." in: Blood, Vol. 106, Issue 10, pp. 3567-74, 2005 (PubMed).

Rieux-Laucat, Hivroz, Lim et al.: "Inherited and somatic CD3zeta mutations in a patient with T-cell deficiency." in: The New England journal of medicine, Vol. 354, Issue 18, pp. 1913-21, 2006 (PubMed).