Intercellular Adhesion Molecule 1 (ICAM1) antibody (Biotin)

Antigen: Intercellular Adhesion Molecule 1 (ICAM1)

Synonyms: BB2, CD54, P3.58, Ly-47, Icam-1, MALA-2, MGC6195, ICAM-1, ICAM1, ICAM

Clonality: Monoclonal (15-Feb)

Host: Mouse

Reactivity: Human

Conjugate: Biotin

Application: Flow Cytometry (FACS)

Catalog no.: ABIN234691

Additional Information

Alternative name: CD54

Immunogen: Rheumatoid synovial cells and human monocytes

Format: Liquid

Isotype: IgG1

Clone: 15-Feb

Description: MAb to CD54 ICAM-1 Monoclonal Antibody to CD54 Biotin conjugated

Specificity: Recognizes the (Mr 85-115kDa) intercellular adhesion molecule ICAM-1 which permits antigen-independent adhesion between lymphocytes and their targets. The LFA-1 binding site is located in domain 1 of ICAM-1. ICAM-1 is a membrane glycoprotein with a wide tissue distribution that includes vascular endothelium and many cells of the immune system. CD54 is weakly expressed on resting peripheral blood lymphocytes. Upon activation by mitogens, the CD54 antigen is strongly expressed on B-cells, T-cells, macrophages and granulocytes. CD54 is the receptor for rhinoviruses and malaria

Application Details

Application Notes: Anti-CD54 (ICAM-1) may be used to study cell-cell interactions. May also be used in studies of rhinovirus and malaria receptors. We recommend using 1ug to stain 1 x 106 cells in flow cytometric applications. Each laboratory should determine an optimum working titer for use in its particular application. Other applications have not been tested but use in such assays should not necessarily be excluded.

Concentration: 100ug/ml (OD280nm)

Purification: Protein G chromatography

Buffer: 0.01M PBS, pH 7.2 containing 1% BSA. Preservative: 0.09% Sodium azide

Storage: Store at 2-8°C. DO NOT FREEZE.

Research Area: CD Antigens, Surface Receptors of Immune Cells, Immunology, Inflammation, Infection

Restrictions: For Research Use only

Publications

Publications: Berendt, McDowall, Craig et al.: "The binding site on ICAM-1 for Plasmodium falciparum-infected erythrocytes overlaps, but is distinct from, the LFA-1-binding site." in: Cell, Vol. 68, Issue 1, pp. 71-81, 1992 (PubMed).