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Neural Cell Adhesion Molecule 1 (NCAM1) antibody

Antigen

Neural Cell Adhesion Molecule 1 (NCAM1)
Synonyms CD56, NCAM, MSK39, ncam, fj56g09, wu:fj56g09, NCAM1, n-cam, LOC397761
Clonality Monoclonal (MEM-188)
Host
Alternatives

Mouse
Reactivity
Alternatives

Human
Conjugate
Alternatives Un-conjugated
Application
Alternatives Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Flow Cytometry (FACS), Immunoprecipitation (IP)
5 references available
Catalog no. ABIN234699
Quantity 100ug  (Lot specific)  (Variants)
Price 235.40 $   Plus shipping costs $45.00
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Alternative name CD56
Immunogen KG-1 human acute myelogenous leukemia cell line
Format Liquid
Isotype IgG2a
Clone MEM-188
Description MAb to CD56 Monoclonal Antibody to Human CD56
Specificity Reacts with a 180 kDa isoform of CD56 (NCAM) characteristic for leukocytes. CD56 is a single transmembrane glycoprotein (immunoglobulin supergene family), the molecular weights of different isoforms ranging from 135 to 220 kDa. Reacts with Human and non-human primates.

Application Details
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Application Notes Suitable for use in Flow cytometry Immunoprecipitation and Immunohistochemistry (paraffin sections, dilution 1:50, heat retrieval (microwave oven) in citrate buffer). Each laboratory should determine an optimum working titer for use in its particular application. Other applications have not been tested but use in such assays should not necessarily be excluded.
Concentration Lot specific
Purification Protein A chromatography. The purified antibody is conjugated with Biotin-LC-NHS under optimum conditions. Product is free of unconjugated biotin. Source: Ascites
Buffer PBS, pH 7.4. Preservative: 15mM Sodium azide
Storage Short term store at 2-8°C. Long term, aliquot and store at -20°C. Avoid multiple freeze/thaw cycles.
Restrictions For Research Use only

Publications
Application Details Alternatives Variants back to top

Brdicka, Imrich, Angelisovua et al.: "Non-T cell activation linker (NTAL): a transmembrane adaptor protein involved in immunoreceptor signaling." in: The Journal of experimental medicine, Vol. 196, Issue 12, pp. 1617-26, 2002 (PubMed).

Brdicková, Brdicka, Angelisová et al.: "LIME: a new membrane Raft-associated adaptor protein involved in CD4 and CD8 coreceptor signaling." in: The Journal of experimental medicine, Vol. 198, Issue 10, pp. 1453-62, 2003 (PubMed).

Lin, Liu, Chen et al.: "CD94 1A transcripts characterize lymphoblastic lymphoma/leukemia of immature natural killer cell origin with distinct clinical features." in: Blood, Vol. 106, Issue 10, pp. 3567-74, 2005 (PubMed).

Drbal, Moertelmaier, Holzhauser et al.: "Single-molecule microscopy reveals heterogeneous dynamics of lipid raft components upon TCR engagement." in: International immunology, Vol. 19, Issue 5, pp. 675-84, 2007 (PubMed).

Olsen, Tollefsen, Aagaard et al.: "Isolation of Mycobacterium avium subspecies paratuberculosis reactive CD4 T cells from intestinal biopsies of Crohn's disease patients." in: PLoS ONE, Vol. 4, Issue 5, pp. e5641, 2009 (PubMed).

Alternatives
Application Details Publications Variants back to top

Alternatives for antigen "Neural Cell Adhesion Molecule 1 (NCAM1)", type "Antibodies"
Hosts Mouse (205), Rabbit (57), Rat (3), Goat (2)
Reactivities Human (252), Mouse (Murine) (51), Rat (Rattus) (51), Pig (Porcine) (39), Chicken (38), Cow (Bovine) (37), Dog (Canine) (37), Horse (Equine) (36), Primate (26), Guinea Pig (18), Zebrafish (Danio rerio) (2), Cat (Feline) (1)
Applications Flow Cytometry (FACS) (174), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (71), Western Blotting (WB) (70), Immunofluorescence (IF) (60), ELISA (46), Immunoprecipitation (IP) (39), Immunohistochemistry (IHC) (25), Immunocytochemistry (ICC) (24), Immunohistochemistry (Fixed) (IHC (fx)) (24), Immunohistochemistry (Frozen Sections) (IHC (fro)) (19), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (8), Immunoelectron Microscopy (IEM) (2), Dot Blot (DB) (1), Functional Studies (Func) (1)
Conjugates Biotin (24), FITC (22), PE (21), APC (8), RPE (5), Alexa Fluor 647 (4), PerCP (3), Alexa Fluor 350 (2), Alexa Fluor 488 (2), Alexa Fluor 555 (2), Cy3 (2), Cy5 (2), Cy5,PE (2), Cy5.5 (2), Cy7 (2), DyLight 649 (2), Dyomics 647 (2), Gold (2), HRP (2), PE,Cy3 (2), PE,Cy5 (2), PE,Cy5.5 (2), PE,Cy7 (2), PE-Dyomics 647 (2), Cy5.5,PE (1), PE-Cy5 (1), PerCP-Cy5.5 (1), SPRD (1)
Epitopes C-Term (3), Extracellular Domain (2), Center (1), Internal Region (1), Internal Region,Isoform 2 (1)