Neural Cell Adhesion Molecule 1 (NCAM1) antibody

Details for Product No. ABIN234699
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Antigen
Synonyms NCAM1, ncam, n-cam, CD56, MSK39, NCAM, E-NCAM, NCAM-1, Ncam, Cd56, N-CAM, N-CAM-1, NCAM-C, NCAMC, fj56g09, ncam1, wu:fj56g09
Reactivity
Human
(333), (63), (58), (39), (38), (36), (35), (17), (6), (6), (3), (2), (1)
Host
Mouse
(280), (73), (4), (2), (1), (1)
Clonality (Clone)
Monoclonal ()
Conjugate
Un-conjugated
(29), (26), (24), (10), (8), (4), (4), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application
Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP)
(213), (110), (74), (54), (52), (36), (33), (28), (28), (20), (5), (3), (1), (1), (1)
Pubmed 5 references available
Quantity 100 μg
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Catalog No. ABIN234699
241.27 $
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Immunogen KG-1 human acute myelogenous leukemia cell line
Clone MEM-188
Isotype IgG2a
Specificity Reacts with a 180 kDa isoform of CD56 (NCAM) characteristic for leukocytes. CD56 is a single transmembrane glycoprotein (immunoglobulin supergene family), the molecular weights of different isoforms ranging from 135 to 220 kDa. Reacts with Human and non-human primates.
Characteristics MAb to CD56, Monoclonal antibody to Human CD56
Purification Protein A Chromatography. The purified antibody is conjugated with Biotin-LC-NHS under optimum conditions. Product is free of unconjugated biotin. Ascites
Alternative Name CD56
Research Area CD Antigens, Surface Receptors of Immune Cells, Signaling
Application Notes Suitable for use in Flow cytometry Immunoprecipitation and Immunohistochemistry (paraffin sections, dilution 1:50, heat retrieval (microwave oven) in citrate buffer). Each laboratory should determine an optimum working titer for use in its particular application. Other applications have not been tested but use in such assays should not necessarily be excluded.
Restrictions For Research Use only
Format Liquid
Buffer PBS, pH 7.4, 15 mM Sodium azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid multiple freeze/thaw cycles.
Centrifuge product if not completely clear after standing at room temperature.
Prepare working dilution only prior to immediate use.
Storage 4 °C/-20 °C
Storage Comment Short term store at 2-8 °C. Long term, aliquot and store at -28 °C.
General Brdicka, Imrich, Angelisová et al.: "Non-T cell activation linker (NTAL): a transmembrane adaptor protein involved in immunoreceptor signaling." in: The Journal of experimental medicine, Vol. 196, Issue 12, pp. 1617-26, 2002 (PubMed).

Brdicková, Brdicka, Angelisová et al.: "LIME: a new membrane Raft-associated adaptor protein involved in CD4 and CD8 coreceptor signaling." in: The Journal of experimental medicine, Vol. 198, Issue 10, pp. 1453-62, 2003 (PubMed).

Lin, Liu, Chen et al.: "CD94 1A transcripts characterize lymphoblastic lymphoma/leukemia of immature natural killer cell origin with distinct clinical features." in: Blood, Vol. 106, Issue 10, pp. 3567-74, 2005 (PubMed).

Drbal, Moertelmaier, Holzhauser et al.: "Single-molecule microscopy reveals heterogeneous dynamics of lipid raft components upon TCR engagement." in: International immunology, Vol. 19, Issue 5, pp. 675-84, 2007 (PubMed).

Olsen, Tollefsen, Aagaard et al.: "Isolation of Mycobacterium avium subspecies paratuberculosis reactive CD4 T cells from intestinal biopsies of Crohn's disease patients." in: PLoS ONE, Vol. 4, Issue 5, pp. e5641, 2009 (PubMed).

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