CD56 (NCAM) antibody (Biotin)

Antigen: CD56 (NCAM)

Clonality: Monoclonal (ERIC-1)

Host: Mouse

Reactivity: Human

Conjugate: Biotin

Application: Flow Cytometry (FACS)

Catalog no.: ABIN234701

Additional Information

Alternative name: CD56 NCAM

Immunogen: Human reinoblastoma tumor tissue, membrane fraction.

Format: Liquid

Isotype: IgG1

Clone: ERIC-1

Description: MAb to CD56 NCAM Monoclonal Antibody to CD56 NCAM Biotin conjugated

Specificity: Recognizes the (Mr 180, 145, 125kDa) isoforms of the human neural cell adhesion molecule (NCAM)(1). The CD56 antigen is expressed on most neuroectodermal derived cell lines, tissues, and tumors. NCAM is also known to be expressed on some mesodermally derived tissues such as muscle (1) and on natural killer (NK) lymphocytes. The binding of ERIC-1 to both normal and neoplastic tissue is lost when tissues are conventionally fixed in formalin and embedded in paraffin. The epitope was preserved when exposed to dehydrating fixatives such as cold acetone (-20? C) for 5 minutes. CD56 is present on 10-25% of peripheral blood lymphocytes.

Application Details

Application Notes: Can be used in studies of the neural cell adhesion molecule. May also be used to study isoforms of NCAM expressed on different tumor types. Works well in western blot for analysis of isoforms expressed. Did not react with any of the leukemias or lymphomas tested (1). We recommend using 1ug to stain 6 x 105 cells in flow cytometric applications. Not for use in IHC with formalin-fixed, paraffin embedded sections. Each laboratory should determine an optimum working titer for use in its particular application. Other applications have not been tested but use in such assays should not necessarily be excluded.

Concentration: 100ug/ml (OD280nm)

Buffer: 0.01M PBS, pH 7.2, containing 2.0mM EDTA, 1% BSA. Preservative: 0.1% Sodium Azide

Storage: Store at 2-8°C. DO NOT FREEZE.

Restrictions: For Research Use only