CD82 (CD83) antibody
| Antigen |
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| Synonyms |
BL11, HB15, R2, 4F9, C33, IA4, ST6, GR15, KAI1, SAR2, TSPAN27 |
| Clonality |
Monoclonal (HB15A) |
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Host
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Reactivity
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Application
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| Catalog no. |
ABIN234754 |
| Quantity |
0.2mg (Not applicable) |
| Price |
463.33 $ Plus shipping costs $35.00
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| Shipping to |
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| Availability |
Ships within 5 to 7 Business Days |
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Alternative name
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CD83
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Immunogen
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COS cells transfected with the HB15 cDNA
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Format
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Purified, Lyophilized
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Isotype
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IgG2b, TBD (Matching secondary antibodies)
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Clone
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HB15A
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Description
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MAb to CD83 Monoclonal Antibody to CD83
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Specificity
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The molecular weight (Mr) of the recognized antigen is 40-45kDa. The CD83 antigen is a 186-aminio acid single-chain glycoprotein. This molecule is a member of the immunoglobulin superfamily and is composed of any extracellular V-type Ig-like single domain, a transmembrane region, and a short, 40-smino-acid cytoplasmic tail. This antibody has been assigned to the CD83 cluster of differentiation during the 5th International Workshop on Human Leucocyte Differentiation Antigens in Boston, U.S.A., in 1993 (4).
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Application Notes
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Research studies by fluorescent microscopy or flow cytometry, after dendritic cells enrichment by culture, enumeration and/or sorting of CD83+ cells. Studies of human dendritic cell subpopulations in tissues by immunohistochemistry. Each laboratory should determine an optimum working titer for use in its particular application. Other applications have not been tested but use in such assays should not necessarily be excluded.
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Concentration
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Not applicable
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Purification
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Ion exchange or affinity chromatography. Source: Ascites
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Buffer
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Lyophilized from PBS containing 1mg/ml BSA.
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Storage
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Store lyophilized product at 2-8°C. After reconstitution, aliquot and store at -20°C. Avoid multiple freeze/thaw cycles. The addition of 0.1% (w/v) sodium azide is recommended for storage of the reconstituted form for up to one month at 2-8°C.Reconstitute with 1ml of distilled water.
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Research Area
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Immunology, CD Antigens, Adaptive Immunity, Surface Receptors of Immune Cells
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Restrictions
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For Research Use only
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Alternatives for antigen "CD82 (CD83)", type "Antibodies"
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Hosts
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Mouse (40), Rabbit (12), Rat (5)
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Reactivities
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Human (50), Mouse (Murine) (9), Primate (3), Rat (Rattus) (3)
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Applications
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Flow Cytometry (FACS) (34), Western Blotting (WB) (20), Enzyme Immunoassay (EIA) (9), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (9), Immunohistochemistry (Frozen Sections) (IHC (fro)) (7), Immunohistochemistry (IHC) (6), ELISA (3), ELISA (Detection) (3), Immunofluorescence (IF) (2), Immunoprecipitation (IP) (1)
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Conjugates
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FITC (7), PE (6), Biotin (4), RPE (1)
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Epitopes
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C-Term (3)
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Alternatives 
