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Antibodies
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anti-Collagen, Type I (COL1) antibody
Collagen, Type I (COL1) antibody
| Antigen |
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| Clonality |
Monoclonal (BDI314) |
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Host
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Reactivity
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Human (70), Rat (Rattus) (30), Mouse (Murine) (27), Cow (Bovine) (25), Dog (Canine) (19), Horse (Equine) (18), Sheep (Ovine) (16), Rabbit (15), Chicken (8), Fish (3), Amphibian (2), Bird (Avian) (2), Mammalian (2), Pig (Porcine) (2)
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Application
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 Alternatives Dot Blot (Dot), ELISA, Immunohistochemistry (Frozen Sections) (IHC (fro))
ELISA (77), Immunohistochemistry (Frozen Sections) (IHC (fro)) (56), Immunofluorescence (IF) (53), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (33), Western Blotting (WB) (24), Flow Cytometry (FACS) (18), Radioimmunoassay (RIA) (18), Immunocytochemistry (ICC) (11), Immunoprecipitation (IP) (9), Immunohistochemistry (IHC) (4), Dot Blot (Dot) (3), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (2), Immunoelectron Microscopy (IEM) (1), Solid Phase Radioimmunoassay (SP-RIA) (1)
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| Catalog no. |
ABIN234846 |
| Quantity |
100ug (Not applicable) |
| Price |
500.67 $ Plus shipping costs $35.00
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| Shipping to |
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| Availability |
Ships within 5 to 7 Business Days |
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Alternative name
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Human Collagen Type I
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Format
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Purified, Lyophilized
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Isotype
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IgG1 (Matching secondary antibodies)
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Clone
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BDI314
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Description
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MAb to Collagen Type I, Human Monoclonal Antibody to Human Collagen Type I Ascites
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Specificity
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Both native and heat denatured human collagen type I. No cross reactivity with albumin fractions of human serum or albumins of other animal species. <5% binding to collagens II and III in ELISA, RIA or immunoblotting.
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Application Notes
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Immunohistochemical staining of 10?m thick cryostat sections fixed with acetone or ethanol. Staining is similar to that for polyclonal antibodies to interstitial collagen I. Suggested working concentration 10-20?g/ml. Formaldehyde fixation is not recommended. Not suitable for use on paraffin embedded sections. ELISA: Apparent binding constant from titration curves is approximately 0.1nM. Suitable for use in sandwich ELISA for specific determination of collagen I, employed as either a capture antibody or as a second, detecting antibody. Does not work with Western blot. Each laboratory should determine an optimum working titer for use in its particular application. Other applications have not been tested but use in such assays should not necessarily be excluded.
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Concentration
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Not applicable
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Purification
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Ammonium sulfate precipitation and chromatography on DEAE-cellulose. Source: Ascites
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Buffer
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Not applicable.
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Storage
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Store (up to 12 months) at -20°C. Avoid multiple freeze/thaw cycles.Reconstitute in 1ml distilled water
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Restrictions
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For Research Use only
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Alternatives for antigen "Collagen, Type I (COL1)", type "Antibodies"
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Hosts
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Rabbit (71), Goat (17), Mouse (15), Bovine (2), Rat (2), Human (1)
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Reactivities
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Human (70), Rat (Rattus) (30), Mouse (Murine) (27), Cow (Bovine) (25), Dog (Canine) (19), Horse (Equine) (18), Sheep (Ovine) (16), Rabbit (15), Chicken (8), Fish (3), Amphibian (2), Bird (Avian) (2), Mammalian (2), Pig (Porcine) (2)
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Applications
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ELISA (77), Immunohistochemistry (Frozen Sections) (IHC (fro)) (56), Immunofluorescence (IF) (53), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (33), Western Blotting (WB) (24), Flow Cytometry (FACS) (18), Radioimmunoassay (RIA) (18), Immunocytochemistry (ICC) (11), Immunoprecipitation (IP) (9), Immunohistochemistry (IHC) (4), Dot Blot (Dot) (3), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (2), Immunoelectron Microscopy (IEM) (1), Solid Phase Radioimmunoassay (SP-RIA) (1)
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Conjugates
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Biotin (11), None (5), Alexa Flour 488 (1), Alexa Fluor 350 (1), Alexa Fluor 488 (1), Alexa Fluor 555 (1), Alexa Fluor 647 (1), Cy3 (1), Cy5 (1), Cy5.5 (1), Cy7 (1), FITC (1), Gold (1), HRP (1), PE (1), PE,Cy3 (1), PE,Cy5 (1), PE,Cy5.5 (1), PE,Cy7 (1), RBITC (1)
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