Cytokeratin 18 (KRT18) antibody

Antigen: Cytokeratin 18 (KRT18)

Synonyms: K18, CK18, Krt1-18, CYK18

Clonality: Monoclonal (Ks18-04)

Host: Mouse

Application: Western Blotting (WB)

Catalog no.: ABIN234925

Additional Information

Alternative name: Cytokeratin 18

Immunogen: Cytokeratin 18 of molecular weight 45kDa. Purified from a HeLa cytoskeletal preparation.

Format: Purified, Lyophilized

Isotype: IgG1  (Matching secondary antibodies)

Clone: Ks18-04

Description: MAb to Cytokeratin 18 Monoclonal Antibody to Cytokeratin 18 Ascites

Specificity: Reacts with the human cytokeratin 18 (45kDa) polypeptide of all simple type epithelia and basal cells of many squamous non-epidermal epithelia. Cross-reacts with mouse, rat, bovine, pig, dog, hamster, sheep, and fish (trout)

Application Details

Application Notes: Western blotting. Suitable for staining formalin-fixed paraffin wax embedded sections following enzyme digestion when incubated for one hour at room temperature. Indirect immunofluorescence or immunoperoxidase staining of frozen sections. Cytological material. Each laboratory should determine an optimum working titer for use in its particular application. Other applications have not been tested but use in such assays should not necessarily be excluded.

Concentration: Not applicable.

Purification: Protein A chromatography. Source: Ascites

Buffer: Lyophilized from PBS, pH 7.4 containing 0.5% BSA. Preservative: 0.09% Sodium Azide

Storage: Short-term (up to 1 month) store at 2-8°C. Long term, aliquot and store at -20°C.Reconstitute with 1ml distilled water.

Research Area: Signaling, Cytoskeleton, Extracellular Matrix

Restrictions: For Research Use only

Publications

Publications: Moll, Franke, Schiller et al.: "The catalog of human cytokeratins: patterns of expression in normal epithelia, tumors and cultured cells." in: Cell, Vol. 31, Issue 1, pp. 11-24, 1983 (PubMed).

Gomi, Yokoyama, Fujimoto et al.: "Mice devoid of the glial fibrillary acidic protein develop normally and are susceptible to scrapie prions." in: Neuron, Vol. 14, Issue 1, pp. 29-41, 1995 (PubMed).

Bantel, Ruck, Gregor et al.: "Detection of elevated caspase activation and early apoptosis in liver diseases." in: European journal of cell biology, Vol. 80, Issue 3, pp. 230-9, 2001 (PubMed).

Stumptner, Fuchsbichler, Heid et al.: "Mallory body--a disease-associated type of sequestosome." in: Hepatology (Baltimore, Md.), Vol. 35, Issue 5, pp. 1053-62, 2002 (PubMed).

Ikenouchi, Matsuda, Furuse et al.: "Regulation of tight junctions during the epithelium-mesenchyme transition: direct repression of the gene expression of claudins/occludin by Snail." in: Journal of cell science, Vol. 116, Issue Pt 10, pp. 1959-67, 2003 (PubMed).

Markl, Strunz-Lehner, Egen-Lappe et al.: "The association of psychosocial factors with nausea and vomiting during pregnancy." in: Journal of psychosomatic obstetrics and gynaecology, pp. 1-6, 2007 (PubMed).