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Keratin 19 (KRT19) antibody

Antigen

Keratin 19 (KRT19)

Synonyms K19, CK19, K1CS, MGC15366, EndoC, Krt1-19, AI663979, Krt-1.19, MGC25344, Ka19, MGC124534, MGC156553, k19, ck19, k1cs, krt9, krt15, MGC76282, KRT19, GK-19, MGC83069, LOC514812, MGC154386, DKFZp469N0613
Clonality Monoclonal (Ks 19-1)
Host
Alternatives

Mouse

Reactivity
Alternatives

Human

Application
Alternatives ELISA, Immunofluorescence (IF), Immunohistochemistry (Frozen Sections) (IHC (fro)), Western Blotting (WB)
Catalog no. ABIN234930
Quantity 50ug  (50ug/ml (prior to lyophilization))
Price 436.67 $   Plus shipping costs $35.00
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Additional Information

Alternative name Cytokeratin 19
Immunogen Cytokeratin 19 of Mr 40,000, from cultured human MCF-7 cells
Format Purified, Lyophilized
Isotype IgG2a  (Matching secondary antibodies)
Clone Ks 19-1
Description MAb to Cytokeratin 19 Monoclonal Antibody to Cytokeratin 19
Specificity Ks 19.1. No reaction with hepatocellular carcinoma. Reactive with cytokeratin 19 rod fragment. Polypeptide reacting: Mr 40,000 polypeptide (cytokeratin 19) of human glandular epithelia. The epitope has been localized on a.a. 311-335 (QSQLSMKAALEDTLAET EARFGAQL) of the alpha-helical fragment. No reaction with hepatocellular carcinoma. Tumors specifically detected: All tested adenocarcinoma, cholangio ca. of liver, renal cell carcinoma, transitional cell ca. of the bladder, ovary ca., squamous cell ca. of cervix, bronchus and lung (intermediate type), mesothelioma, carcinoid tumor of bronchus, breast ca., thymoma.

Application Details

Application Notes Immunohistochemistry on frozen tissue Immunohistochemistry on paraffin-embedded tissues (microwave procedure highly recommended) (dilute 1:10) Immunofluorescence microscopy Western blot ELISA Each laboratory should determine an optimum working titer for use in its particular application. Other applications have not been tested but use in such assays should not necessarily be excluded. FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES. Catalog M44191M Page 2 of 2
Concentration 50ug/ml (prior to lyophilization)
Purification Protein A chromatography. Source: Ascites
Buffer Lyophilized from PBS, pH 7.4 containing 0.5% BSA. Preservative: 0.09% Sodium azide
Storage After reconstitution, short term (up to 1 year) store at 2-8°C. Long term, aliquot and store at -20°C. Avoid multiple freeze/thaw cycles.Reconstitute in 1ml distilled water.
Research Area Signaling, Cytoskeleton, Cytokeratins, Extracellular Matrix
Restrictions For Research Use only

Alternatives

Alternatives for antigen "Keratin 19 (KRT19)", type "Antibodies"
Hosts Mouse (71), Rabbit (31), Guinea Pig (1)
Reactivities Human (87), Rat (Rattus) (12), Mouse (Murine) (11), Cow (Bovine) (3), Monkey (3), None (3), Dog (Canine) (2), Amphibian (1), Chicken (1), Fish (1), Guinea Pig (1)
Applications Western Blotting (WB) (68), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (59), Immunofluorescence (IF) (32), Immunohistochemistry (Frozen Sections) (IHC (fro)) (24), Immunohistochemistry (IHC) (21), Immunoprecipitation (IP) (18), ELISA (17), Enzyme Immunoassay (EIA) (16), Immunocytochemistry (ICC) (10), Flow Cytometry (FACS) (8), ELISA (Detection) (5), Dot Blot (Dot) (1)
Conjugates Biotin (4), Alexa Fluor 488 (1), FITC (1), HRP (1)
Epitopes C-Term (3), AA 311-335 (1), AA 312-335 (1), Center (1), S46 (1), pS46 (1)