Desmocollin 3 (DSC3) antibody

Antigen: Desmocollin 3 (DSC3)

Synonyms: DSC, DSC1, DSC2, DSC4, CDHF3, HT-CP, dg3, DSC3, dsc3, MGC85083

Clonality: Monoclonal (Dsc3-U114)

Host: Mouse

Reactivity: Human

Conjugate: Un-conjugated

Application: Immunohistochemistry (Frozen Sections) (IHC (fro)), Western Blotting (WB)

Catalog no.: ABIN234979

Additional Information

Alternative name: Desmocollin 3

Immunogen: Synthetic peptide corresponding to a sequence present on the extracellular anchor domain of human desmocollin 3 (VHGAPFYFSLPNTSPEISRLWSLTKVC)

Format: Liquid

Isotype: IgG1

Clone: Dsc3-U114

Description: MAb to Desmocollin 3 Monoclonal Antibody to Human Desmocollin 3 Cell culture

Specificity: MAb localizes Dsc3 present in all living epidermal layers, in glandular duct cells, in basal matrix cells and outer root sheath of hair follicles, basal and suprabasal cell layers of stratified epithelia (e.g. vagina, tongue, esophagus), basal layer of bladder urothelium and complex epithelium of trachea, and thymic reticulum cells. All simple epithelia are negative. Intercalated disks of myocardium are negative. Polypeptide reacting: 109kDa and 10 kDa polypeptides (desmocollin 3 splice isoforms at pI 5.2) from human epidermal desmosomes. Most cultured cell lines derived from stratified squamous epithelia or squamous cell carcinomas are positive (e.g. A-431, HaCaT). Specific for human desmocollin 3. Does not react with bovine and rodent.

Application Details

Application Notes: Suitable for Immunohistochemistry on frozen and paraffin-embedded sections after microwave treatment (use undiluted) and Western blot. For better resolution on frozen sections preincubation (directly after fixation) with 0.05 ? 0.2% Triton X100, for 5-10 minutes depending on tissue type, is recommended. Each laboratory should determine an optimum working titer for use in its particular application. Other applications have not been tested but use in such assays should not necessarily be excluded. FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES. Catalog H44350M Page 2 of 2

Concentration: Not determined

Purification: Supernatant Source: Cell culture

Buffer: Not applicable. Preservative: 0.09% Sodium azide

Storage: Store at 2-8°C.

Restrictions: For Research Use only

Publications

Nuber, Schaefer, Stehr et al.: "Patterns of desmocollin synthesis in human epithelia: immunolocalization of desmocollins 1 and 3 in special epithelia and in cultured cells." in: European journal of cell biology, Vol. 71, Issue 1, pp. 1-13, 1997 (PubMed).

Kurzen, Moll, Moll et al.: "Compositionally different desmosomes in the various compartments of the human hair follicle." in: Differentiation; research in biological diversity, Vol. 63, Issue 5, pp. 295-304, 1998 (PubMed).

Moll, Houdek, Schaefer et al.: "Diversity of desmosomal proteins in regenerating epidermis: immunohistochemical study using a human skin organ culture model." in: Archives of dermatological research, Vol. 291, Issue 7-8, pp. 437-46, 1999 (PubMed).

Hatzfeld, Haffner, Schulze et al.: "The function of plakophilin 1 in desmosome assembly and actin filament organization." in: The Journal of cell biology, Vol. 149, Issue 1, pp. 209-22, 2000 (PubMed).

South, Wan, Stone et al.: "Lack of plakophilin 1 increases keratinocyte migration and reduces desmosome stability." in: Journal of cell science, Vol. 116, Issue Pt 16, pp. 3303-14, 2003 (PubMed).

Akat, Mennel, Kremer et al.: "Molecular characterization of desmosomes in meningiomas and arachnoidal tissue." in: Acta neuropathologica, Vol. 106, Issue 4, pp. 337-47, 2003 (PubMed).