Escherichia Coli O157 (E. coli) antibody (FITC)

Antigen: Escherichia Coli O157

Epitope: E. coli

Clonality: Monoclonal (BDI160)

Host: Mouse

Conjugate: FITC

Application: ELISA, Immunofluorescence (IF)

Catalog no.: ABIN235031

Additional Information

Alternative name: Escherichia coli (E. coli) O157

Immunogen: Whole cells

Format: Liquid

Isotype: IgG2b

Clone: BDI160

Description: MAb to E. coli O157 Monoclonal Antibody to Escherichia coli (E. coli) O157 Fluorescein conjugated

Specificity: Specific for E. coli serotype O157. Non-reactive with E. coli serotypes O111, O125, O20, O55 and K12.

Application Details

Application Notes: Suitable for use in Immunofluorescence and ELISA. Acetone fixation of the antigen source is recommended prior to staining. Each laboratory should determine an optimum working titer for use in its particular application. Other applications have not been tested but use in such assays should not necessarily be excluded.

Concentration: 100ug/ml (OD280nm, E0.1% 1.3)

Purification: Conjugated with high purity isomer I of fluorescein isothiocyanate. Care is taken to ensure complete removal of any free fluorescein from the final product. Source: Ascites

Buffer: 0.01M PBS, pH 7.2 containing 10mg/ml BSA. Preservative: 0.1% Sodium azide

Storage: Short term (up to 6 months) store at 2-8°C. Long term, aliquot and store at -20°C. Avoid multiple freeze/thaw cycles.

Restrictions: For Research Use only