Epidermal Growth Factor Receptor (EGFR) (Amino Acids 1165-1186) antibody

Antigen: Epidermal Growth Factor Receptor (EGFR)

Synonyms: ERBB, HER1, mENA, ERBB1, PIG61, Wa5, wa2, Erbb, Errp, wa-2, AI552599, 9030024J15Rik, ErbB-1, C-erb, D-EGFR, D-Egf, DEGFR, DER, DER flb, DER/EGFR, DER/faint little ball, DER/top, DER/torpedo, DmHD-33, EFG-R, EGF-R, EGFR, EK2-6, Egf, El, Elp, Elp-1, Elp-B1, Elp-B1RB1, HD-33, Torpedo/DER, Torpedo/Egfr, c-erbB, d-egf-r, dEGFR1, flb, l(2)05351, l(2)09261, l(2)57DEFa, l(2)57EFa, l(2)57Ea, pnt, top, top/DER, top/flb, DmelCG10079, CG10079, egfr, EGFR12, EGFR15, egfr1, Erbb2

Epitope: Amino Acids 1165-1186

Clonality: Monoclonal (13G8)

Host: Mouse

Reactivity: Human

Application: ELISA, Immunofluorescence (IF), Western Blotting (WB)

Catalog no.: ABIN235070

Additional Information

Alternative name: Human Epidermal Growth Factor Receptor (EGFR), (amino acids 1165-1186)

Immunogen: Peptide conjugated to KLH Format: Purified, Lyophilized Reconstitute with 1ml water for 15 minutes at room temperature.

Format: Purified, Lyophilized

Isotype: IgG1  (Matching secondary antibodies)

Clone: 13G8

Description: Cell lysate of 2 x 106 untreated HepG2 cells.

Specificity: Specifically recognizes the C-terminus of EGF receptor (a.a. 1165-1186). Recognition is independent of the phosphorylation status at tyrosine 1173. Reacts with Human, mouse and rat.

Synonyms: ERBB, HER1, mENA, ERBB1, PIG61, Wa5, wa2, Erbb, Errp, wa-2, AI552599, 9030024J15Rik, ErbB-1, C-erb, D-EGFR, D-Egf, DEGFR, DER, DER flb, DER/EGFR, DER/faint little ball, DER/top, DER/torpedo, DmHD-33, EFG-R, EGF-R, EGFR, EK2-6, Egf, El, Elp, Elp-1, Elp-B1, Elp-B1RB1, HD-33, Torpedo/DER, Torpedo/Egfr, c-erbB, d-egf-r, dEGFR1, flb, l(2)05351, l(2)09261, l(2)57DEFa, l(2)57EFa, l(2)57Ea, pnt, top, top/DER, top/flb, DmelCG10079, CG10079, egfr, EGFR12, EGFR15, egfr1, Erbb2

Application Details

Application Notes: Immunoblotting: 1ug/ml for HRP/ECL detection. Recommended blocking buffer CPPT: 0.5% (w/v) casein, 1% (w/v) PEG 4000, 1% (w/v) Polyvinylpyrrolidone (PVP), 0.1% Tween 20, 10mM Tris/HCl, pH 7.4, 150mM Sodium Chloride. Detection of endogenous EGFR Whole cell lysates of serum starved tumor cells (20,000 cells per lane) were applied to SDS-PAGE and transferred to a PVDF membrane. The blot was probed with 0.5?g/ml K67138M for 1 hour at RT and developed by ECL (exp. time: 30 sec). Lane 1: A431 Lane 5: HaCaT Lane 2: A549 Lane 6: PC3 Lane 3: SKOV3 Lane 7: HeLa Lane 4: OVCAR5 Lane 8: HepG2 ELISA: 0.05ug/ml Immunoprecipitation: 1 ? 10ug per 106 vanadate treated A431 cells. Immunocytochemistry: 0.1 ? 1ug/ml Each laboratory should determine an optimum working titer for use in its particular application. Other applications have not been tested but use in such assays should not necessarily be excluded. Storage: Store lyophilized product at ?20?C. After reconstitution, aliquot and store at ?80?C. Thawed aliquots may be stored at 2-8?C for up to 3 months. Warning:

Concentration: 100ug/ml (prior to lyophilization)

Purification: Thiophilic adsorption and size exclusion chromatography. Source: Cell culture

Buffer: Lyophilized from 2X PBS, PEG and Sucrose. Preservative: 0.09% Sodium Azide

Storage: Store lyophilized product at -20°C. After reconstitution, aliquot and store at -80°C. Thawed aliquots may be stored at 2-8°C for up to 3 months. Warning:Reconstitute with 1ml water for 15 minutes at room temperature. Purification:

Restrictions: For Research Use only

Images

Detection of endogenous EGFR: Whole cell lysates of serum starved tumor cells (20,000 cells per lane) were applied to SDS-PAGE and transferred to a PVDF membrane. The blot was probed with 0.5?g/ml ABIN235070 for 1 hour at RT and developed by ECL (exp. time: 30 sec). Lane 1: A431 Lane 5: HaCaT Lane 2: A549 Lane 6: PC3 Lane 3: SKOV3 Lane 7: HeLa Lane 4: OVCAR5Lane 8: HepG2