Epidermal Growth Factor Receptor (EGFR) (prylated At Tyrosine 1045) antibody

Antigen: Epidermal Growth Factor Receptor (EGFR)

Synonyms: ERBB, HER1, mENA, ERBB1, PIG61, Wa5, wa2, Erbb, Errp, wa-2, AI552599, 9030024J15Rik, ErbB-1, C-erb, D-EGFR, D-Egf, DEGFR, DER, DER flb, DER/EGFR, DER/faint little ball, DER/top, DER/torpedo, DmHD-33, EFG-R, EGF-R, EGFR, EK2-6, Egf, El, Elp, Elp-1, Elp-B1, Elp-B1RB1, HD-33, Torpedo/DER, Torpedo/Egfr, c-erbB, d-egf-r, dEGFR1, flb, l(2)05351, l(2)09261, l(2)57DEFa, l(2)57EFa, l(2)57Ea, pnt, top, top/DER, top/flb, DmelCG10079, CG10079, egfr, EGFR12, EGFR15, egfr1, Erbb2

Epitope: prylated At Tyrosine 1045

Clonality: Monoclonal (11C2)

Host: Mouse

Reactivity: Human

Application: ELISA, Western Blotting (WB)

Catalog no.: ABIN235073

Additional Information

Alternative name: Human Epidermal Growth Factor Receptor (EGFR), phosphorylated at Tyrosine 1045

Immunogen: Phosphopeptide conjugated to KLH Format: Purified, Liquid

Format: Purified, Liquid

Isotype: IgG1  (Matching secondary antibodies)

Clone: 11C2

Description: Cell lysate of pervanadate treated HepG2 cells Cell culture

Specificity: Recognizes EGFR phosphorylated at Tyrosine 1045. Epitope: Phosphotyrosine pY1045 L Q R pY S S D. Reacts with Human and mouse.

Synonyms: ERBB, HER1, mENA, ERBB1, PIG61, Wa5, wa2, Erbb, Errp, wa-2, AI552599, 9030024J15Rik, ErbB-1, C-erb, D-EGFR, D-Egf, DEGFR, DER, DER flb, DER/EGFR, DER/faint little ball, DER/top, DER/torpedo, DmHD-33, EFG-R, EGF-R, EGFR, EK2-6, Egf, El, Elp, Elp-1, Elp-B1, Elp-B1RB1, HD-33, Torpedo/DER, Torpedo/Egfr, c-erbB, d-egf-r, dEGFR1, flb, l(2)05351, l(2)09261, l(2)57DEFa, l(2)57EFa, l(2)57Ea, pnt, top, top/DER, top/flb, DmelCG10079, CG10079, egfr, EGFR12, EGFR15, egfr1, Erbb2

Application Details

Application Notes: Immunoblotting: 0.5ug/ml for HRP/ECL detection. Recommended blocking buffer CPPT: 0.5% (w/v) casein, 1% (w/v) PEG 4000, 1% (w/v) Polyvinylpyrrolidone (PVP), 0.1% Tween 20, 10mM Tris/HCl, pH 7.4, 150mM Sodium chloride. EGFR Activation Serum starved MDA-MB 468 cells were incubated with 10ng/ml EGF for the indicated times. Whole cell lysates were prepared and separated by SDS-PAGE (20,000 cells/lane). The blot was probed with 0.5?g/ml K67112M for 1 hour at RT and developed by ECL (exp. time: 30 sec). Lane 1: control Lane 5: 1 hour EGF Lane 2: 5 min EGF Lane 6: 2 hour EGF Lane 3: 15 min EGF Lane 7: 4 hour EGF Lane 4: 30 min EGF Lane 8: 8 hour EGF Immunoprecipitation: use at 1-10ug per 106 pervanadate-treated A431 cells ELISA: 0.05ug/ml Each laboratory should determine an optimum working titer for use in its particular application. Other applications have not been tested but use in such assays should not necessarily be excluded. Storage: Upon receipt, aliquot and store (up to 1 year) at -20?C. Avoid multiple freeze/thaw cycles. Warning:

Concentration: 100ug/ml

Purification: Thiophilic adsorption and size exclusion chromatography. Source: Cell culture

Buffer: PBS, PEG, Sucrose containing 50% Glycerol. Preservative: 0.09% Sodium azide

Storage: Upon receipt, aliquot and store (up to 1 year) at -20°C. Avoid multiple freeze/thaw cycles. Warning:

Restrictions: For Research Use only

Images

EGFR Activation: Serum starved MDA-MB 468 cells were incubated with 10ng/ml EGF for the indicated times. Whole cell lysates were prepared and separated by SDS-PAGE (20,000 cells/lane). The blot was probed with 0.5?g/ml ABIN235073 for 1 hour at RT and developed by ECL (exp. time: 30 sec). Lane 1: control Lane 5: 1 hour EGF Lane 2: 5 min EGF Lane 6: 2 hour EGF Lane 3: 15 min EGF Lane 7: 4 hour EGF Lane 4: 30 min EGF Lane 8: 8 hour EGF