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Antibody
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anti-Histone H1
Histone H1 antibody
| Antigen |
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| Synonyms |
H1c, H1.2, H1f2, His1a, H1var1, MGC107646, 0610008C09Rik, LOC692942, HISTONE H1, HON4 |
| Clonality |
Monoclonal (AE-4) |
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Host
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| Catalog no. |
ABIN235436 |
| Quantity |
2ml (Not determined) |
| Price |
290.00 $ Plus shipping costs $35.00
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| Shipping to |
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| Availability |
Ships within 5 to 10 Business Days |
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Format
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Supernatant, Liquid
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Isotype
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IgG2a (Matching secondary antibodies)
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Clone
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AE-4
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Description
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MAb to Histone H1 Monoclonal Antibody to Histone H1
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Specificity
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Recognizes histone H1 in the nucleus of animal and plant cells. Using purified histone fractions monoclonal AE-4 has been shown to have preferential binding to histone H1 by immunoblotting and ELISA methods.
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Application Notes
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Produces a speckled staining pattern in the nucleus of normal and malignant cells and may be used to stain nuclear histones. We highly recommend fixing cells with 2.0% paraformaldehyde. Tonsil is the recommended positive control tissue. We suggest using this product without dilution. Each laboratory should determine an optimum working titer for use in its particular application. Other applications have not been tested but use in such assays should not necessarily be excluded.
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Concentration
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Not determined
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Purification
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Not applicable. Source: Tissue culture supernatant
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Buffer
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Filtered tissue culture supernatant (RPMI-1640), pH 7.2. Preservative: 0.09% Sodium azide
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Storage
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Short term (up to one month) store at 2-8°C. Long-term, aliquot and store below -20°C. Avoid multiple freeze/thaw cycles.
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Research Area
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Chromatin and Nuclear Signaling, DNA/RNA, Transcription Factors
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Restrictions
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For Research Use only
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Alternatives for antigen "Histone H1", type "Antibodies"
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Hosts
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Rabbit (27), Mouse (18), Sheep (5), Chicken (4)
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Reactivities
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Human (41), Cow (Bovine) (22), Mouse (Murine) (18), Dog (Canine) (15), Horse (Equine) (15), Pig (Porcine) (15), Rabbit (15), Rat (Rattus) (15), Mammalian (1)
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Applications
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Immunofluorescence (IF) (33), Western Blotting (WB) (31), ELISA (21), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (18), Flow Cytometry (FACS) (13), Immunohistochemistry (IHC) (10), Immunoprecipitation (IP) (8), Immunohistochemistry (Frozen Sections) (IHC (fro)) (6), Immunocytochemistry (ICC) (3), Enzyme Immunoassay (EIA) (2), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (2), Immunoelectron Microscopy (EM) (1)
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Conjugates
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Alexa Flour 488 (1), Biotin (1), Cy3 (1), Cy5 (1), Cy5.5 (1), Cy7 (1), FITC (1), Gold (1), HRP (1), PE (1), PE,Cy3 (1), PE,Cy5 (1), PE,Cy7 (1), RBITC (1)
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Epitopes
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Acetyl-Lys25 (2), AA 100-200 (1), C-Term (1), N-Term (1), pThr17 (1)
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Alternatives 
