Mouse anti-Human Immunoglobulin A, Secretory Component (sIgA) Antibody

Antigen: Immunoglobulin A, Secretory Component (sIgA)

Clonality: Monoclonal (B781M)

Host: Mouse

Reactivity: Human

Conjugate: Un-conjugated

Application: ELISA

Catalog no.: ABIN235559

Additional Information

Alternative name: IgA, Secretory Component

Immunogen: Purified Human IgA

Format: Purified, Lyophilized

Isotype: IgG1

Clone: B781M

Description: MAb to Human IgA (Secretory) Monoclonal Antibody to Human Immunoglobulin A (IgA), Secretory Component

Specificity: Human IgA Secretory Component. Reacts against all human IgA subtypes.

Application Details

Application Notes: Suitable for use in ELISA. By direct ELISA, 1:20,000 dilution will yield >1.0 O.D. using alkaline phosphatase conjugated Rabbit anti-mouse Ig. Each laboratory should determine an optimum working titer for use in its particular application. Other applications have not been tested but use in such assays should not necessarily be excluded. Reconstitution: Reconstitute with water to a concentration of 1mg/ml. Mix gently, wash the sides of the vial and wait 30-60 seconds before use.

Concentration: 1mg/ml (prior to lyophilization)

Purification: Ion exchange column chromatography

Buffer: Lyophilized from PBS Preservative: None

Storage: Store lyophilized product at 2-8°C. After reconstitution, short term (up to 1 month) store at 2-8°C. Long term, aliquot and store at –20°C. Avoid multiple freeze/thaw cycles..

Restrictions: For Research Use only

Publications

Publications: Kappler, Krauss-Etschmann, Diehl et al.: "Detection of secretory IgA antibodies against gliadin and human tissue transglutaminase in stool to screen for coeliac disease in children: validation study." in: BMJ (Clinical research ed.), Vol. 332, Issue 7535, pp. 213-4, 2006 (PubMed).

Oortwijn, van der Boog, Roos et al.: "A pathogenic role for secretory IgA in IgA nephropathy." in: Kidney international, Vol. 69, Issue 7, pp. 1131-8, 2006 (PubMed).

Lin, Zhao, Yan: "[The influence of alexithymia in stress on secretory IgA and cortisol in saliva]" in: Shanghai kou qiang yi xue = Shanghai journal of stomatology, Vol. 14, Issue 6, pp. 561-4, 2006 (PubMed).

Hoshino, Shoji, Inada et al.: "[Clinical evaluation of a measurement method for secretory IgA in tears]" in: Nippon Ganka Gakkai zasshi, Vol. 110, Issue 4, pp. 276-81, 2006 (PubMed).

Uehara, Sano, Yarita et al.: "[Penicillium marneffei isolated from a Thai AIDS patient with fungemia]" in: Nihon Ishinkin Gakkai zasshi = Japanese journal of medical mycology, Vol. 49, Issue 3, pp. 205-9, 2008 (PubMed).