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Antibodies
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anti-Interferon gamma (IFNG) antibody
Interferon gamma (IFNG) antibody
| Antigen |
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| Synonyms |
IFG, IFI, Ifg, IFN-g, IFNG2, IFNG, IFN-gamma |
| Clonality |
Monoclonal (IF-H12) |
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Host
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Application
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Western Blotting (WB) (103), Flow Cytometry (FACS) (77), ELISA (61), Enzyme Immunoassay (EIA) (52), Immunofluorescence (IF) (47), Immunoprecipitation (IP) (19), Functional Studies (Func) (16), Immunohistochemistry (IHC) (12), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (9), Blocking Antibody (Inhibition) (8), Immunohistochemistry (Frozen Sections) (IHC (fro)) (8), Intracellular Staining (IS) (7), ELISA (Detection) (6), Neutralization (Neut) (5), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (4), ELISA (Capture) (3), Dot Blot (Dot) (2), Immunoelectron Microscopy (IEM) (2), ELISPOT (1), Radioimmunoassay (RIA) (1)
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| Catalog no. |
ABIN235707 |
| Quantity |
0.2mg (1mg/ml (prior to lyophilization)) |
| Price |
195.33 $ Plus shipping costs $35.00
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| Shipping to |
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| Availability |
Ships within 5 to 7 Business Days |
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Alternative name
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Human Interferon gamma (IFN-gamma)
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Immunogen
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Recombinant Human INF-gamma
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Format
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Purified, Lyophilized
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Isotype
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IgG (Matching secondary antibodies)
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Clone
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IF-H12
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Description
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MAb to Human IFN-gamma Monoclonal Antibody to Human Interferon gamma (IFN-gamma)
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Specificity
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IFN-gamma
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Application Notes
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Neutralizing and binding (ELISA) antibody. This antibody only works in a capture ELISA assay. It does not recognize plastic-bound IFN-gamma, but works very well in capture ELISA when used as the first, plastic bound antibody (and then developed with polyclonal antibody). Titer in capture ELISA, using 100ng/well immobilized MAb, 100 pg of IFN-gamma will give a reading of 0.15 O.D. Neutralization titers vary with the virus assay used. Each laboratory should determine an optimum working titer for use in its particular application. Other applications have not been tested but use in such assays should not necessarily be excluded.
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Concentration
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1mg/ml (prior to lyophilization)
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Purification
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Protein A/S-Sepharose. Source: Ascites
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Buffer
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Lyophilized from PBS.
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Storage
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Store lyophilized product (up to 2 years) at -20°C. After reconstitution, if not used within a month, aliquot and store at -20°C.Reconstitute with 0.2ml water. Mix gently, wash the sides of the vial and wait 30-60 seconds before use.
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Restrictions
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For Research Use only
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Alternatives for antigen "Interferon gamma (IFNG)", type "Antibodies"
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Hosts
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Mouse (89), Rabbit (85), Rat (14), Goat (6), Chicken (1), Sheep (1)
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Reactivities
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Human (113), Mouse (Murine) (48), Rat (Rattus) (37), Cow (Bovine) (11), Monkey (9), Pig (Porcine) (7), Dog (Canine) (5), Horse (Equine) (5), Sheep (Ovine) (5), Goat (4), Chicken (3), Rabbit (2), Cat (Feline) (1)
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Applications
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Western Blotting (WB) (103), Flow Cytometry (FACS) (77), ELISA (61), Enzyme Immunoassay (EIA) (52), Immunofluorescence (IF) (47), Immunoprecipitation (IP) (19), Functional Studies (Func) (16), Immunohistochemistry (IHC) (12), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (9), Blocking Antibody (Inhibition) (8), Immunohistochemistry (Frozen Sections) (IHC (fro)) (8), Intracellular Staining (IS) (7), ELISA (Detection) (6), Neutralization (Neut) (5), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (4), ELISA (Capture) (3), Dot Blot (Dot) (2), Immunoelectron Microscopy (IEM) (2), ELISPOT (1), Radioimmunoassay (RIA) (1)
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Conjugates
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Biotin (16), FITC (9), PE (6), Alexa Fluor 350 (3), Alexa Fluor 488 (3), Alexa Fluor 555 (3), Alexa Fluor 647 (3), PE,Cy5.5 (3), APC (2), Alexa Flour 488 (2), Cy3 (2), Cy5 (2), Cy5.5 (2), Cy7 (2), Gold (2), HRP (2), PE,Cy3 (2), PE,Cy5 (2), PE,Cy7 (2), RBITC (2), RPE (1)
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Epitopes
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C-Term peptide (6)
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Alternatives 
