Filamentous Phages M13 Fd F1 antibody (Biotin)

Antigen: Filamentous Phages M13 Fd F1

Clonality: Monoclonal (B62-FE2)

Host: Mouse

Conjugate: Biotin

Application: ELISA, Western Blotting (WB)

Catalog no.: ABIN235800

Additional Information

Alternative name: Filamentous Phages, M13, fd, F1

Immunogen: fd phages from E. coli F+ strain (JM109)

Format: Liquid

Isotype: IgG2b

Clone: B62-FE2

Description: MAb to Filamentous Phages M13/fd/F1 Monoclonal Antibody to Filamentous Phages, M13, fd, F1 Biotin conjugated

Specificity: Binds to an epitope on pVIII (phage coat protein) covering the N-terminal region of g8p AEGDDPAKAAFDSLQASAT.

Application Details

Application Notes: Phage Display (immunoassays for the identification of recombinant antigen or antibody phages) Immunoblotting ELISA (1:5,000?1:10,000) Each laboratory should determine an optimum working titer for use in its particular application. Other applications have not been tested but use in such assays should not necessarily be excluded.

Concentration: 100ug/ml

Purification: Protein A chromatography. Source: Tissue culture

Buffer: PBS, pH 7.4 containing 0.5% BSA. Preservative: 0.09% Sodium azide

Storage: Store at 2-8°C.

Restrictions: For Research Use only

Publications

Publications: Micheel, Fiebach, Karsten et al.: "Monoclonal antibodies to different epitopes of human alpha-fetoprotein (AFP)." in: European journal of cancer & clinical oncology, Vol. 19, Issue 9, pp. 1239-46, 1983 (PubMed).

Kneissel, Queitsch, Petersen et al.: "Epitope structures recognised by antibodies against the major coat protein (g8p) of filamentous bacteriophage fd (Inoviridae)." in: Journal of molecular biology, Vol. 288, Issue 1, pp. 21-8, 1999 (PubMed).

Rondot, Koch, Breitling et al.: "A helper phage to improve single-chain antibody presentation in phage display." in: Nature biotechnology, Vol. 19, Issue 1, pp. 75-8, 2001 (PubMed).