Nitric Oxide Synthase 3 (Endothelial Cell) (NOS3) (pSer1177) antibody

Antigen: Nitric Oxide Synthase 3 (Endothelial Cell) (NOS3)

Synonyms: NOS, INOS, NOS2A, HEP-NOS, iNOS, Nos-2, Nos2a, i-NOS, NOS-II, eNOS, Nos-3, ecNOS, 2310065A03Rik, iNos, cNOS, EC-NOS, NOSIII, NOS3, NOS2, nos2, ECNOS

Epitope: pSer1177

Clonality: Monoclonal (BDI250)

Host: Mouse

Application: ELISA, Western Blotting (WB)

Catalog no.: ABIN235945

Additional Information

Alternative name: Nitric Oxide Synthase, Endothelial (eNOS) phosphorylated serine 1177 (pSer1177)

Immunogen: Synthetic phosphopeptide corresponding to amino acid residues surrounding S1177

Format: Purified, Liquid

Isotype: IgG1, kappa chain  (Matching secondary antibodies)

Clone: BDI250

Description: MAb to eNOS (pSer1177) Monoclonal Antibody to Nitric Oxide Synthase, Endothelial (eNOS) phosphorylated serine 1177 (pSer1177)

Specificity: Endothelial NO synthase produces nitric oxide (NO) which plays a crucial role in cardiovascular homeostasis. Activation of the enzyme and NO production can be induced by phosphorylation of eNOS at Serine 1177 by activated Akt/PKB. Specifically recognizes eNOS phosphorylated at serine 1177. MW 140kDa. Cross-reacts with human and mouse.

Application Details

Application Notes: Western blot: 2ug/ml for HRP/ECL detection Western blot Analysis Whole cell lysates of untreated (lane 1) and pervanadate-treated (lane 2) Bend 3 cells were separated by SDS-PAGE and transferred to a PVDF membrane. The blot was probed with 1?g/ml Q67250M for 1 hour at 15-22?C and developed by ECL (exp. time: 3 min). Q67250M recognizes eNOS pS 1177 at 140 kDa. ELISA: use at 0.1ug/ml Each laboratory should determine an optimum working titer for use in its particular application. Other applications have not been tested but use in such assays should not necessarily be excluded.

Concentration: 100ug/ml

Purification: Thiophilic adsorption and size exclusion chromatography. Source: Cell culture

Buffer: 2X PBS, PEG, Sucrose and 50% glycerol. Preservative: 0.09% Sodium azide

Storage: Aliquot and store at -20°C. Avoid multiple freeze/thaw cycles.

Restrictions: For Research Use only

Images

Western blot Analysis: Whole cell lysates of untreated (lane 1) and pervanadate-treated (lane 2) Bend 3 cells were separated by SDS-PAGE and transferred to a PVDF membrane. The blot was probed with 1?g/ml ABIN235945 for 1 hour at 15-22ºC and developed by ECL (exp. time: 3 min). ABIN235945 recognizes eNOS pS 1177 at 140 kDa.