Perforin 1 (Pore Forming Protein) (PRF1) antibody

Antigen: Perforin 1 (Pore Forming Protein) (PRF1)

Synonyms: PRF1, P1, PFP, FLH2, PFN1, HPLH2, MGC65093, Pfn, Pfp, Prf-1, Cyta, RATCYTA, MGC108712, LOC443187, perforin, prf1

Clonality: Monoclonal (4E4)

Host: Mouse

Reactivity: Human

Conjugate: Un-conjugated

Application: ELISA, Flow Cytometry (FACS)

Catalog no.: ABIN235998

Additional Information

Alternative name: Perforin

Format: Liquid

Isotype: IgG2a

Clone: 4E4

Description: MAb to Perforin Monoclonal Antibody to Human Perforin

Specificity: Human Perforin

Application Details

Application Notes: Suitable for use in Flow cytometry and ELISA. Each laboratory should determine an optimum working titer for use in its particular application. Other applications have not been tested but use in such assays should not necessarily be excluded.

Concentration: 0.5mg/ml (OD280nm)

Purification: Affinity chromatography on human Type I collagen covalently linked to agarose. Cross absorption on collagen types II, III, IV, V and VI covalently linked to agarose.

Buffer: 100mM Borate buffered saline, pH 8.2.

Storage: Store at 2-8°C.

Restrictions: For Research Use only

Publications

Hameed, Olsen, Cheng et al.: "Immunohistochemical identification of cytotoxic lymphocytes using human perforin monoclonal antibody." in: The American journal of pathology, Vol. 140, Issue 5, pp. 1025-30, 1992 (PubMed).

Lichtenheld, Olsen, Lu et al.: "Structure and function of human perforin." in: Nature, Vol. 335, Issue 6189, pp. 448-51, 1988 (PubMed).

Masson, Tschopp: "Isolation of a lytic, pore-forming protein (perforin) from cytolytic T-lymphocytes." in: The Journal of biological chemistry, Vol. 260, Issue 16, pp. 9069-72, 1985 (PubMed).

Lowin, Hahne, Mattmann et al.: "Cytolytic T-cell cytotoxicity is mediated through perforin and Fas lytic pathways." in: Nature, Vol. 370, Issue 6491, pp. 650-2, 1994 (PubMed).

Braun, Lowin, French et al.: "Cytotoxic T cells deficient in both functional fas ligand and perforin show residual cytolytic activity yet lose their capacity to induce lethal acute graft-versus-host disease." in: The Journal of experimental medicine, Vol. 183, Issue 2, pp. 657-61, 1996 (PubMed).

Smyth, Thia, Cretney et al.: "Perforin is a major contributor to NK cell control of tumor metastasis." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 162, Issue 11, pp. 6658-62, 1999 (PubMed).

Rode, Balkow, Sobek et al.: "Perforin and Fas act together in the induction of apoptosis, and both are critical in the clearance of lymphocytic choriomeningitis virus infection." in: Journal of virology, Vol. 78, Issue 22, pp. 12395-405, 2004 (PubMed).

Gupta, Greer, Mahanty et al.: "CD8-mediated protection against Ebola virus infection is perforin dependent." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 174, Issue 7, pp. 4198-202, 2005 (PubMed).