Phosphotyrosine (Set of 5 MAbs) antibody

Antigen: Phosphotyrosine (Set of 5 MAbs)

Clonality: Monoclonal (SETTYR)

Host: Mouse

Application: ELISA, Immunoprecipitation (IP), Western Blotting (WB)

Catalog no.: ABIN236044

Additional Information

Immunogen: Phosphopeptide conjugated to KLH

Format: Purified, Lyophilized

Clone: SETTYR

Description: Phosphotyrosine (Set of 5 MAbs) Set Includes: Catalog #ABIN236050 Clone BDI820 Catalog #ABIN236048 Clone BDI230 Catalog #ABIN236051 Clone BDI890 Catalog #ABIN236049 Clone BDI460 Catalog #ABIN236045 Clone BDI512

Specificity: Phosphotyrosine Detection kit combines 25ug of each of the monoclonal anti-phosphotyrosine antibodies and a Phosphotyrosine Molecular Weight Marker.

Application Details

Application Notes: Western blot: 1ug/ml for AP/BCIP/NBT (MTT) detection, 0.1ug/ml for HRP/ECL detection. Immunoprecipitation: use at 1-10ug per 106 vanadate treated A431 cells. ELISA: use at 0.05-0.1ug/ml Each laboratory should determine an optimum working titer for use in its particular application. Other applications have not been tested but use in such assays should not necessarily be excluded. Catalog #K67700MLysates of pervanadate-treatedA431 cells were probed with: Lane 1: ABIN236045 (1ug/ml)Lane 2: ABIN236050 (1ug/ml)Lane 3: ABIN236048 (1ug/ml)Lane 4: ABIN236051 (1ug/ml)Lane 5: ABIN236049 (1ug/ml) Reconstitution: Reconstitute with 1ml water per vial for 15 minutes at room temperature.

Concentration: Not applicable.

Purification: Thiophilic adsorption and size exclusion chromatography.

Buffer: Lyophilized from 2X PBS, PEG and Sucrose. Preservative: 0.09% Sodium Azide .Catalog #K67700MPage 2 of 3

Storage: Store lyophilized product at –20°C. After reconstitution, aliquot and store at –80°C. Thawed aliquots may be stored at 2-8°C for up to 3 months. Avoid multiple freeze/thaw cycles.Warning: This product contains sodium azide, which has been classified as Xn (Harmful), in European Directive 67/548/EEC in the concentration range of 0.1 – 1.0 %. When disposing of this reagent through lead or copper plumbing, flush with copious volumes of water to prevent azide build-up in drains.Includes Positive Control:

Restrictions: For Research Use only

Images

Lysates of pervanadate-treated. A431 cells were probed with: Lane 1: ABIN236045 (1ug/ml). Lane 2: ABIN236050 (1ug/ml). Lane 3: ABIN236048 (1ug/ml). Lane 4: ABIN236051 (1ug/ml). Lane 5: ABIN236049 (1ug/ml).