Podoplanin (PDPN) antibody

Antigen: Podoplanin (PDPN)

Synonyms: T1A, GP36, GP40, Gp38, OTS8, T1A-2, AGGRUS, HT1A-1, PA2.26, T1a, OTS-8, T1alpha, RANDAM-2, E11, RTI40, T1-alpha, PDPN

Clonality: Monoclonal (4D5aE5E6)

Host: Mouse

Reactivity: Human

Conjugate: Un-conjugated

Application: Immunohistochemistry (Frozen Sections) (IHC (fro))

Catalog no.: ABIN236093

Additional Information

Alternative name: Podoplanin

Immunogen: ORF Human Podoplanin ectodomain expressed in E. coli

Format: Liquid

Isotype: IgG1

Clone: 4D5aE5E6

Description: MAb to Podoplanin Monoclonal Antibody to Human Podoplanin

Specificity: Recognizes Human Podoplanin (gp36), a 36kDa type I cell membrane-associated glycoprotein, with a broad spectrum of distribution, being strongly expressed on the lymphatic endothelium found in lung, placenta and skeletal muscle and to a lesser extent in brain, kidney and liver. Podoplanin is absent on blood endothelium and therefore this antibody is able to distinguish between lymphatic vessels and blood vasculature.

Application Details

Application Notes: Suitable for use in Immunohistochemistry, frozen and paraffin sections (0.75?2ug/ml). This product requires antigen retrieval using heat treatment prior to staining of paraffin sections. Sodium citrate buffer pH 6.0 is recommended for this purpose. Each laboratory should determine an optimum working titer for use in its particular application. Other applications have not been tested but use in such assays should not necessarily be excluded.

Concentration: 1mg/ml

Purification: Ion exchange chromatography

Buffer: PBS. Preservative: 0.09% Sodium azide

Storage: Short term store at 2-8°C. Long term store at -20°C. Avoid multiple freeze/thaw cycles.

Restrictions: For Research Use only

Publications

Zimmer, Oeffner, Von Messling et al.: "Cloning and characterization of gp36, a human mucin-type glycoprotein preferentially expressed in vascular endothelium." in: The Biochemical journal, Vol. 341 ( Pt 2), pp. 277-84, 1999 (PubMed).

Dadras, Paul, Bertoncini et al.: "Tumor lymphangiogenesis: a novel prognostic indicator for cutaneous melanoma metastasis and survival." in: The American journal of pathology, Vol. 162, Issue 6, pp. 1951-60, 2003 (PubMed).

Schacht, Ramirez, Hong et al.: "T1alpha/podoplanin deficiency disrupts normal lymphatic vasculature formation and causes lymphedema." in: The EMBO journal, Vol. 22, Issue 14, pp. 3546-56, 2003 (PubMed).

Kato, Fujita, Kunita et al.: "Molecular identification of Aggrus/T1alpha as a platelet aggregation-inducing factor expressed in colorectal tumors." in: The Journal of biological chemistry, Vol. 278, Issue 51, pp. 51599-605, 2003 (PubMed).

Misaki, Matsui, Matsuda: "Metabolic enzyme induction by HepG2 cells exposed to oxygenated and nonoxygenated polycyclic aromatic hydrocarbons." in: Chemical research in toxicology, Vol. 20, Issue 2, pp. 277-83, 2007 (PubMed).