anti-Podoplanin antibody (PDPN)

Details for Product anti-PDPN Antibody No. ABIN236093
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Synonyms PDPN, AGGRUS, GP36, GP40, Gp38, HT1A-1, OTS8, PA2.26, T1A, T1A-2, OTS-8, RANDAM-2, T1-alpha, T1a, T1alpha, E11, RTI40
(199), (64), (14), (3)
(105), (71), (31), (25), (14), (13), (4), (2)
Clonality (Clone)
Monoclonal ()
This Podoplanin antibody is un-conjugated
(17), (14), (9), (8), (7), (6), (6), (5), (3), (2), (1), (1), (1), (1), (1), (1), (1), (1)
Immunohistochemistry (Formalin-fixed Sections) (IHC (f)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
(141), (67), (66), (62), (41), (29), (28), (15), (11), (6), (5), (5), (3), (3), (3), (2), (2), (2), (1), (1), (1), (1)
Pubmed 5 references available
Quantity 100 μg
Shipping to United States ( )
Availability Will be delivered in 4 to 5 Business Days
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Immunogen ORF Human Podoplanin ectodomain expressed in E. coli
Clone 4D5aE5E6
Isotype IgG1
Specificity Recognizes Human Podoplanin (gp36), a 36 kDa type I cell membrane-associated glycoprotein, with a broad spectrum of distribution, being strongly expressed on the lymphatic endothelium found in lung, placenta and skeletal muscle and to a lesser extent in brain, kidney and liver. Podoplanin is absent on blood endothelium and therefore this antibody is able to distinguish between lymphatic vessels and blood vasculature.
Characteristics MAb to Podoplanin, Monoclonal antibody to Human Podoplanin
Purification Ion Exchange Chromatography
Alternative Name Podoplanin (PDPN Antibody Abstract)
Application Notes Suitable for use in Immunohistochemistry, frozen and paraffin sections (0.75-2 µg/mL). This product requires antigen retrieval using heat treatment prior to staining of paraffin sections. Sodium citrate buffer pH 6.0 is recommended for this purpose. Each laboratory should determine an optimum working titer for use in its particular application. Other applications have not been tested but use in such assays should not necessarily be excluded.
Restrictions For Research Use only
Format Liquid
Buffer PBS, 0.09 % Sodium azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid multiple freeze/thaw cycles.
Centrifuge product if not completely clear after standing at room temperature.
Prepare working dilution only prior to immediate use.
Storage 4 °C/-20 °C
Storage Comment Short term store at 2-8 °C. Long term store at -28 °C.
Background publications Misaki, Matsui, Matsuda: "Metabolic enzyme induction by HepG2 cells exposed to oxygenated and nonoxygenated polycyclic aromatic hydrocarbons." in: Chemical research in toxicology, Vol. 20, Issue 2, pp. 277-83, 2007 (PubMed).

Kato, Fujita, Kunita et al.: "Molecular identification of Aggrus/T1alpha as a platelet aggregation-inducing factor expressed in colorectal tumors." in: The Journal of biological chemistry, Vol. 278, Issue 51, pp. 51599-605, 2003 (PubMed).

Dadras, Paul, Bertoncini et al.: "Tumor lymphangiogenesis: a novel prognostic indicator for cutaneous melanoma metastasis and survival." in: The American journal of pathology, Vol. 162, Issue 6, pp. 1951-60, 2003 (PubMed).

General Schacht, Ramirez, Hong et al.: "T1alpha/podoplanin deficiency disrupts normal lymphatic vasculature formation and causes lymphedema." in: The EMBO journal, Vol. 22, Issue 14, pp. 3546-56, 2003 (PubMed).

Zimmer, Oeffner, Von Messling et al.: "Cloning and characterization of gp36, a human mucin-type glycoprotein preferentially expressed in vascular endothelium." in: The Biochemical journal, Vol. 341 ( Pt 2), pp. 277-84, 1999 (PubMed).

Validation Images
Catalog No. ABIN236093
$ 740.67
Plus shipping costs $45.00
100 μg
$ 740.67

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