Scramblase 1 (N-Term) antibody

Antigen: Scramblase 1

Synonyms: CG6590, CT20492, Scramb1, DmelCG32056, CG32056

Epitope: N-Term

Clonality: Monoclonal (1-00E-09)

Host: Mouse

Reactivity: Human

Application: ELISA, Western Blotting (WB)

Catalog no.: ABIN236335

Additional Information

Alternative name: Human Scramblase-1, N-terminal

Immunogen: Peptide conjugated to KLH Format: Purified, Lyophilized Reconstitute with 1ml water for 15 minutes at room temperature.

Format: Purified, Lyophilized

Isotype: IgG1  (Matching secondary antibodies)

Clone: 1-00E-09

Description: Cell lysate of serum starved untreated HepG2 cells

Specificity: Scramblase-1 is a calcium-binding protein that can be induced by interferon and growth factors (e.g. the Epidermal Growth Factor EGF). Depending on its palmitoylation state, Scramblase-1 either inserts into the plasma membrane or binds DNA in the nucleus. Scramblase-1 is a substrate of src kinases. Clone 1E9 specifically recognizes the N-terminus of Scramblase-1 at 35 kDa. Does not react with mouse, rat or dog.

Application Details

Application Notes: Western blot: 1ug/ml for HRP/ECL detection. Recommended blocking buffer CPPT: 0.5% (w/v) casein, 1% (w/v) PEG 4000, 1% (w/v) Polyvinylpyrrolidone (PVP), 0.1% Tween 20, 10mM Tris/HCl, pH 7.4, 150mM Sodium Chloride. Detection of endogenous scramblase 1 Whole cell lysates of serum starved tumor cells were applied to SDS-PAGE and transferred to a PVDF membrane. The blot was probed with 0.5ug/ml H67109M for 1 hour at RT and developed by ECL (exp. time: 30 sec). Lane 1: HeLa Lane 5, Jurkat Lane 2: HepG2 Lane 6: U937 Lane 3: HEK-293 Lane 7: K562 Lane 4: SY5Y Lane 8: THP Each laboratory should determine an optimum working titer for use in its particular application. Other applications have not been tested but use in such assays should not necessarily be excluded. Storage: Store lyophilized product at -20?C. After reconstitution, aliquot and store at -80?C. Thawed aliquots may be stored at 2?8?C for up to 3 months. Avoid multiple freeze/thaw cycles. Warning:

Concentration: 100ug/ml (prior to lyophilization)

Purification: Thiophilic adsorption and size exclusion chromatography. Source: Cell culture

Buffer: Lyophilized from 2X PBS, PEG and Sucrose. Preservative: 0.09% Sodium azide

Storage: Store lyophilized product at -20°C. After reconstitution, aliquot and store at -80°C. Thawed aliquots may be stored at 2-8°C for up to 3 months. Avoid multiple freeze/thaw cycles. Warning:Reconstitute with 1ml water for 15 minutes at room temperature. Purification:

Restrictions: For Research Use only

Images

Detection of endogenous scramblase 1: Whole cell lysates of serum starved tumor cells were applied to SDS-PAGE and transferred to a PVDF membrane. The blot was probed with 0.5ug/ml ABIN236335 for 1 hour at RT and developed by ECL (exp. time: 30 sec). Lane 1: HeLa Lane 2: HepG2 Lane 3: HEK-293 Lane 4: SY5Y Lane 5, Jurkat Lane 6: U937 Lane 7: K562 Lane 8: THP