Spectrin Binding Protein, Hssh3bp1 (SH3 Domain) antibody

Antigen: Spectrin Binding Protein, Hssh3bp1

Binding Site: SH3 Domain

Clonality: Monoclonal (4E2)

Host: Mouse

Application: Immunofluorescence (IF), Immunohistochemistry (Frozen Sections) (IHC (fro)), Western Blotting (WB)

Catalog no.: ABIN236347

Additional Information

Immunogen: Recombinant Hssh3bp1 (human spectrin SH3 domain binding protein 1)

Format: Liquid

Isotype: IgG2b

Clone: 4E2

Description: MAb to Spectrin Binding Protein Monoclonal Antibody to Spectrin Binding Protein, SH3 domain Hssh3bp1

Specificity: Cross-reacts with Human, rat and mouse. This antibody was developed against the spectrin SH3 domain binding protein Hssh3bp1(3). Hssh3bp1 is a marker of macropinocytic vesicles and a potential regulator of macropinocytosis.(2) The gene encoding Hssh3bp1 has a role as a tumor suppressor gene which is inactivated during prostate tumorigenesis.(1)

Application Details

Application Notes: Western blot (1:1,000 ? 1:2,000) Immunofluorescence (1:100 ? 1:500) Immunohistochemistry (1:40 ? 1:100) Formalin-fixed, paraffin-embedded sections and frozen sections. For optimal staining, sections should be pre-treated for antigen unmasking. Each laboratory should determine an optimum working titer for use in its particular application. Other applications have not been tested but use in such assays should not necessarily be excluded.

Concentration: 1mg/ml

Purification: Protein A chromatography

Buffer: PBS.

Storage: Store at -20°C. Aliquot to avoid multiple freeze/thaw cycles.

Restrictions: For Research Use only

Publications

Bennett, Gilligan: "The spectrin-based membrane skeleton and micron-scale organization of the plasma membrane." in: Annual review of cell biology, Vol. 9, pp. 27-66, 1994 (PubMed).

Ziemnicka-Kotula, Xu, Gu et al.: "Identification of a candidate human spectrin Src homology 3 domain-binding protein suggests a general mechanism of association of tyrosine kinases with the spectrin-based membrane skeleton." in: The Journal of biological chemistry, Vol. 273, Issue 22, pp. 13681-92, 1998 (PubMed).

De Matteis, Morrow: "Spectrin tethers and mesh in the biosynthetic pathway." in: Journal of cell science, Vol. 113 ( Pt 13), pp. 2331-43, 2000 (PubMed).

Xu, Ziemnicka, Merz et al.: "Human spectrin Src homology 3 domain binding protein 1 regulates macropinocytosis in NIH 3T3 cells." in: Journal of cell science, Vol. 113 Pt 21, pp. 3805-14, 2000 (PubMed).

Macoska, Xu, Ziemnicka et al.: "Loss of expression of human spectrin src homology domain binding protein 1 is associated with 10p loss in human prostatic adenocarcinoma." in: Neoplasia (New York, N.Y.), Vol. 3, Issue 2, pp. 99-104, 2001 (PubMed).