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Spectrin, alpha-I (Erythroid) (SH3 Domain) antibody

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SH3 Domain
Clonality (Clone)
Monoclonal ()
ELISA, Indirect Immunofluorescence Assay (IFA), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)), Western Blotting (WB)
Pubmed 4 references available
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Quantity 0.2 mL
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Immunogen Recombinant alpha I spectrin (erythroid spectrin) SH3 domain
Clone 17C7
Isotype IgG1
Cross-Reactivity (Details) This antibody was developed against the alpha I spectrin SH3 domain.(2) Specific marker for human, rat and mouse alpha I spectrin. Reacts with erythroid spectrin in red blood cells and in cerebellum. Does not cross react with alpha II (non-erythroid) spectrin SH3 domain or recombinant human fodrin SH3 domain. In cerebellar cell cultures, clone 17C7 labels cell bodies consistent with localization of erythroid spectrin. In NIH 3T3 cells, clone 17C7 reacts with a 200kDa protein which is a candidate marker of endocytic vesicles called macropinosomes.(1,2,5)
Characteristics MAb to Alpha-I Spectrin (erythroid), Monoclonal antibody to Human Spectrin, alpha-I SH3 domain (erythroid), Tissue culture supernatant
Purification HPLC Purified. Ascites
Application Notes ELISA (1:105 - 1:106) Western blot (1:102 - 1:103) Immunofluorescence (1:100 - 1:500) Immunohistochemistry (1:10 - 1:50) Frozen only Each laboratory should determine an optimum working titer for use in its particular application. Suitability for use in Immunoprecipitation has not been determined but use in such assays should not necessarily be excluded.
Restrictions For Research Use only
Format Liquid
Buffer PBS, 0.1 % Sodium Azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid multiple freeze/thaw cycles.
Centrifuge product if not completely clear after standing at room temperature.
Prepare working dilution only prior to immediate use.
Storage -20 °C
Storage Comment Store at -20 °C.
Background publications Xu, Ziemnicka, Scalia et al.: "Monoclonal antibodies to alphaI spectrin Src homology 3 domain associate with macropinocytic vesicles in nonerythroid cells." in: Brain research, Vol. 898, Issue 1, pp. 171-7, 2001 (PubMed).

Xu, Ziemnicka, Merz et al.: "Human spectrin Src homology 3 domain binding protein 1 regulates macropinocytosis in NIH 3T3 cells." in: Journal of cell science, Vol. 113 Pt 21, pp. 3805-14, 2000 (PubMed).

De Matteis, Morrow: "Spectrin tethers and mesh in the biosynthetic pathway." in: Journal of cell science, Vol. 113 ( Pt 13), pp. 2331-43, 2000 (PubMed).

Bennett, Gilligan: "The spectrin-based membrane skeleton and micron-scale organization of the plasma membrane." in: Annual review of cell biology, Vol. 9, pp. 27-66, 1994 (PubMed).

Catalog No. ABIN236348

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