Spectrin, alpha-I (erythroid) (SH3 Domain) antibody

Antigen: Spectrin, alpha-I (erythroid)

Binding Site: SH3 Domain

Clonality: Monoclonal (17C7)

Host: Mouse

Reactivity: Human

Application: ELISA, Immunofluorescence (IF), Immunohistochemistry (Frozen Sections) (IHC (fro)), Western Blotting (WB)

Catalog no.: ABIN236348

Additional Information

Immunogen: Recombinant alpha I spectrin (erythroid spectrin) SH3 domain

Format: Liquid

Isotype: IgG1

Clone: 17C7

Description: MAb to Alpha-I Spectrin (erythroid) Monoclonal Antibody to Human Spectrin, alpha-I SH3 domain (erythroid) Tissue culture supernatant

Specificity: This antibody was developed against the alpha I spectrin SH3 domain.(2) Specific marker for human, rat and mouse alpha I spectrin. Reacts with erythroid spectrin in red blood cells and in cerebellum. Does not cross react with alpha II (non-erythroid) spectrin SH3 domain or recombinant human fodrin SH3 domain. In cerebellar cell cultures, clone 17C7 labels cell bodies consistent with localization of erythroid spectrin. In NIH 3T3 cells, clone 17C7 reacts with a 200kDa protein which is a candidate marker of endocytic vesicles called macropinosomes.(1,2,5)

Application Details

Application Notes: ELISA (1:105 ? 1:106) Western blot (1:102 ? 1:103) Immunofluorescence (1:100 ? 1:500) Immunohistochemistry (1:10 ? 1:50) Frozen only Each laboratory should determine an optimum working titer for use in its particular application. Suitability for use in Immunoprecipitation has not been determined but use in such assays should not necessarily be excluded. FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES. Catalog Q06177M Page 2 of 2

Concentration: 1mg/ml

Purification: HPLC purified. Source: Ascites

Buffer: PBS. Preservative: 0.1% Sodium Azide

Storage: Store at -20°C. Aliquot to avoid multiple freeze/thaw cycles.

Restrictions: For Research Use only

Publications

Bennett, Gilligan: "The spectrin-based membrane skeleton and micron-scale organization of the plasma membrane." in: Annual review of cell biology, Vol. 9, pp. 27-66, 1994 (PubMed).

De Matteis, Morrow: "Spectrin tethers and mesh in the biosynthetic pathway." in: Journal of cell science, Vol. 113 ( Pt 13), pp. 2331-43, 2000 (PubMed).

Xu, Ziemnicka, Merz et al.: "Human spectrin Src homology 3 domain binding protein 1 regulates macropinocytosis in NIH 3T3 cells." in: Journal of cell science, Vol. 113 Pt 21, pp. 3805-14, 2000 (PubMed).

Xu, Ziemnicka, Scalia et al.: "Monoclonal antibodies to alphaI spectrin Src homology 3 domain associate with macropinocytic vesicles in nonerythroid cells." in: Brain research, Vol. 898, Issue 1, pp. 171-7, 2001 (PubMed).