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Details for Product No. ABIN236349

Spectrin, alpha-II (Non-Erythroid) (Fodrin) (SH3 Domain) antibody

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Antigen
Epitope
»Alternatives SH3 Domain
Reactivity
Human
Host
»Alternatives Mouse
Clonality (Clone) Monoclonal ()
Application
»Alternatives ELISA, Immunofluorescence (IF), Immunohistochemistry (Frozen Sections) (IHC (fro)), Western Blotting (WB)
Pubmed 5 references available
Catalog no. ABIN236349
Quantity 100 µL
Price
1,135.93 $   Plus shipping costs $45.00 and if applicable $26.40 dry ice
Shipping to
Availability Will be delivered in 4 to 5 Business Days
Immunogen Recombinant alpha II spectrin (non-erythroid spectrin or fodrin) SH3 domain.
Clone D8B7
Cross-Reactivity (Details) This antibody was developed against the alpha II spectrin SH3 domain.(1) Specific marker for human, rat and mouse alpha II spectrin or fodrin. In cerebellar cell cultures, it labels axons and periphery of cell bodies consistent with localization of fodrin.(3,5,7) Does not cross-react with alpha I (erythroid) spectrin SH3 domain.
Characteristics MAb to Alpha-II Spectrin (non-erythroid), Monoclonal antibody to Human Spectrin, alpha-II SH3 domain (non-erythroid) (fodrin)
Purification HPLC Purified. Ascites
Application Notes ELISA (1:100.000 - 1:1.000,000) Western blot (1:100 - 1:1.000) Immunofluorescence (1:100 - 1:500) Immunohistochemistry (1:10 - 1:50) Frozen sections (paraffin-embedded sections have not been tested) Each laboratory should determine an optimum working titer for use in its particular application. Other applications have not been tested but use in such assays should not necessarily be excluded.
Restrictions For Research Use only
Format Liquid
Buffer PBS, 0.1% Sodium azide
Preservative Sodium azide
Handling Advice Avoid multiple freeze/thaw cycles.
Storage -20 °C
Storage Comment Store at -20°C.
Goodman, Zimmer, Clark et al.: "Brain spectrin: of mice and men." in: Brain research bulletin, Vol. 36, Issue 6, pp. 593-606, 1995 (PubMed).

Bennett, Gilligan: "The spectrin-based membrane skeleton and micron-scale organization of the plasma membrane." in: Annual review of cell biology, Vol. 9, pp. 27-66, 1994 (PubMed).

De Matteis, Morrow: "Spectrin tethers and mesh in the biosynthetic pathway." in: Journal of cell science, Vol. 113 ( Pt 13), pp. 2331-43, 2000 (PubMed).

Xu, Ziemnicka, Merz et al.: "Human spectrin Src homology 3 domain binding protein 1 regulates macropinocytosis in NIH 3T3 cells." in: Journal of cell science, Vol. 113 Pt 21, pp. 3805-14, 2000 (PubMed).

Xu, Ziemnicka, Scalia et al.: "Monoclonal antibodies to alphaI spectrin Src homology 3 domain associate with macropinocytic vesicles in nonerythroid cells." in: Brain research, Vol. 898, Issue 1, pp. 171-7, 2001 (PubMed).

Alternatives for antigen "Spectrin, alpha-II (Non-Erythroid) (Fodrin)", type "Antibodies"
Hosts (1)
Applications (1), (1), (1), (1)
Epitopes (1)
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