Spectrin, alpha-II (non-erythroid) (fodrin) (SH3 Domain) antibody

Antigen: Spectrin, alpha-II (non-erythroid) (fodrin)

Binding Site: SH3 Domain

Clonality: Monoclonal (D8B7)

Host: Mouse

Reactivity: Human

Application: ELISA, Immunofluorescence (IF), Immunohistochemistry (Frozen Sections) (IHC (fro)), Western Blotting (WB)

Catalog no.: ABIN236349

Additional Information

Immunogen: Recombinant alpha II spectrin (non-erythroid spectrin or fodrin) SH3 domain.

Format: Liquid

Isotype: IgG2b

Clone: D8B7

Description: MAb to Alpha-II Spectrin (non-erythroid) Monoclonal Antibody to Human Spectrin, alpha-II SH3 domain (non-erythroid) (fodrin)

Specificity: This antibody was developed against the alpha II spectrin SH3 domain.(1) Specific marker for human, rat and mouse alpha II spectrin or fodrin. In cerebellar cell cultures, it labels axons and periphery of cell bodies consistent with localization of fodrin.(3,5,7) Does not cross-react with alpha I (erythroid) spectrin SH3 domain.

Application Details

Application Notes: ELISA (1:100,000 ? 1:1,000,000) Western blot (1:100 ? 1:1,000) Immunofluorescence (1:100 ? 1:500) Immunohistochemistry (1:10 ? 1:50) Frozen sections (paraffin-embedded sections have not been tested) Each laboratory should determine an optimum working titer for use in its particular application. Other applications have not been tested but use in such assays should not necessarily be excluded.

Concentration: Not determined

Purification: HPLC purified. Source: Ascites

Buffer: PBS. Preservative: 0.1% Sodium azide

Storage: Store at -20°C. Aliquot to avoid multiple freeze/thaw cycles.

Restrictions: For Research Use only

Publications

Goodman, Zimmer, Clark et al.: "Brain spectrin: of mice and men." in: Brain research bulletin, Vol. 36, Issue 6, pp. 593-606, 1995 (PubMed).

Bennett, Gilligan: "The spectrin-based membrane skeleton and micron-scale organization of the plasma membrane." in: Annual review of cell biology, Vol. 9, pp. 27-66, 1994 (PubMed).

De Matteis, Morrow: "Spectrin tethers and mesh in the biosynthetic pathway." in: Journal of cell science, Vol. 113 ( Pt 13), pp. 2331-43, 2000 (PubMed).

Xu, Ziemnicka, Merz et al.: "Human spectrin Src homology 3 domain binding protein 1 regulates macropinocytosis in NIH 3T3 cells." in: Journal of cell science, Vol. 113 Pt 21, pp. 3805-14, 2000 (PubMed).

Xu, Ziemnicka, Scalia et al.: "Monoclonal antibodies to alphaI spectrin Src homology 3 domain associate with macropinocytic vesicles in nonerythroid cells." in: Brain research, Vol. 898, Issue 1, pp. 171-7, 2001 (PubMed).