T-Cell Receptor V beta-5.2 antibody

Antigen: T-Cell Receptor V beta-5.2

Clonality: Monoclonal (36213)

Host: Mouse

Application: Flow Cytometry (FACS)

Catalog no.: ABIN236447

Additional Information

Immunogen: 1C1 V beta 5.2 positive cell line.

Format: Liquid

Isotype: IgG1  (Matching secondary antibodies)

Clone: 36213

Description: MAb to T-Cell Rec V beta-5.2 Monoclonal Antibody to T-Cell Receptor V beta-5.2 Phycoerythrin conjugated Ascites

Specificity: Human variable beta 5.2 chain of the T-cell receptor also called TCRBV5S2 according to the nomenclature from Wei et al.(1). This antibody recognizes only the PL2.5 sequence (2). It does not recognize the V beta 5.1 [HPB51 sequence (3) and the V beta 5.3 (IGRb08 sequence (4)]. Recognition of other members of the V beta 5 family has not been detected but cannot be formally excluded. Cell sorting on PBL gives a heterogenous pattern of staining of the sorted cells. The antibody may thus be sensitive to the clonotypic environment (V alpha, J alpha, D beta, J beta) though molecular analysis of transcripts from the sorted V beta 5.2 positive cells shows defferent V alpha, D and J beta (5). The specificity of this antibody has been confirmed at the First Human TcR Monoclonal Antibody Workshop in San Francisco in 1995.(6)

Application Details

Application Notes: T-cell repertoire studies Flow cytometry: 20ul/5 x 105 cells/test or 100ul whole blood Each laboratory should determine an optimum working titer for use in its particular application. Other applications have not been tested but use in such assays should not necessarily be excluded.

Concentration: 12.5ug/ml

Purification: Source: Ascites

Buffer: PBS containing 2mg/ml BSA. Preservative: 0.1% Sodium Azide

Storage: Store (in the dark) at 2-8(C. DO NOT FREEZE.

Restrictions: For Research Use only

Publications

Publications: Ferradini, Roman-Roman, Azocar et al.: "Studies on the human T cell receptor alpha/beta variable region genes. II. Identification of four additional V beta subfamilies." in: European journal of immunology, Vol. 21, Issue 4, pp. 935-42, 1991 (PubMed).

Concannon, Pickering, Kung et al.: "Diversity and structure of human T-cell receptor beta-chain variable region genes." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 83, Issue 17, pp. 6598-602, 1986 (PubMed).

Kimura, Toyonaga, Yoshikai et al.: "Sequences and diversity of human T cell receptor beta chain variable region genes." in: The Journal of experimental medicine, Vol. 164, Issue 3, pp. 739-50, 1986 (PubMed).

Wei, Charmley, Robinson et al.: "The extent of the human germline T-cell receptor V beta gene segment repertoire." in: Immunogenetics, Vol. 40, Issue 1, pp. 27-36, 1994 (PubMed).

Diu, Romagne, Genevee et al.: "Fine specificity of monoclonal antibodies directed at human T cell receptor variable regions: comparison with oligonucleotide-driven amplification for evaluation of V beta expression." in: European journal of immunology, Vol. 23, Issue 7, pp. 1422-9, 1993 (PubMed).