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Details for Product No. ABIN236474

Coagulation Factor II (thrombin) Receptor (F2R) (N-Term), (AA 51-64) antibody

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Synonyms TR, HTR, CF2R, PAR1, PAR-1, Par1, TRGPC, AI482343, Cf2r, ThrR, Par-1, f2r-a, par1
»Alternatives N-Term, AA 51-64
»Alternatives Mouse
Clonality (Clone) Monoclonal ()
»Alternatives Un-conjugated
»Alternatives Flow Cytometry (FACS), Immunoprecipitation (IP), Western Blotting (WB)
Pubmed 5 references available
Catalog no. ABIN236474
Quantity 0.2 mg
568.33 $   Plus shipping costs $45.00
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Availability Will be delivered in 4 to 5 Business Days
Immunogen N-terminal peptide of thrombin receptor residues 51-64 (KYEPFWEDEEKNES)
Clone B825M
Specificity Recognizes an epitope on the receptor, distant from the N-terminal thrombin cleavage site (WEDEE). It reacts with both intact and cleaved receptors.
Characteristics MAb to Thrombin Receptor, Monoclonal antibody to Thrombin Receptor
Purification Ascites
Alternative Name Thrombin Receptor
Research Area Chromatin and Nuclear Signaling, Cell Cycle, Angiogenesis, Coagulation
Application Notes Suitable for use in Western blot, immunoprecipitation and Flow cytometry (2 µg/5x105 cells or 2 µg/100ul whole blood). Each laboratory should determine an optimum working titer for use in its particular application. Other applications have not been tested but use in such assays should not necessarily be excluded.
Restrictions For Research Use only
Format Lyophilized
Reconstitution Reconstitute with 1mL distilled water.
Concentration 0.2 mg/mL (prior to lyophilization)
Buffer Lyophilized from PBS containing 1mg/ml BSA.
Handling Advice Avoid multiple freeze/thaw cycles. The addition of 0.1% (w/v) sodium azide is recommended for storage (up to one month) at 2-8°C.
Storage 4 °C/-20 °C
Storage Comment Store lyophilized product at 2-8°C. After reconstitution, aliquot and store at -20°C.
Brass, Vassallo, Belmonte et al.: "Structure and function of the human platelet thrombin receptor. Studies using monoclonal antibodies directed against a defined domain within the receptor N terminus." in: The Journal of biological chemistry, Vol. 267, Issue 20, pp. 13795-8, 1992 (PubMed).

Vu, Hung, Wheaton et al.: "Molecular cloning of a functional thrombin receptor reveals a novel proteolytic mechanism of receptor activation." in: Cell, Vol. 64, Issue 6, pp. 1057-68, 1991 (PubMed).

Woolkalis, DeMelfi, Blanchard et al.: "Regulation of thrombin receptors on human umbilical vein endothelial cells." in: The Journal of biological chemistry, Vol. 270, Issue 17, pp. 9868-75, 1995 (PubMed).

Molino, Blanchard, Belmonte et al.: "Proteolysis of the human platelet and endothelial cell thrombin receptor by neutrophil-derived cathepsin G." in: The Journal of biological chemistry, Vol. 270, Issue 19, pp. 11168-75, 1995 (PubMed).

Hoxie, Ahuja, Belmonte et al.: "Internalization and recycling of activated thrombin receptors." in: The Journal of biological chemistry, Vol. 268, Issue 18, pp. 13756-63, 1993 (PubMed).

Alternatives for antigen "Coagulation Factor II (thrombin) Receptor (F2R)", type "Antibodies"
Hosts (56), (17), (8), (1)
Reactivities (79), (17), (15)
Applications (63), (37), (19), (10), (7), (7), (3), (3), (2), (2), (1), (1)
Conjugates (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Epitopes (23), (4), (4), (4), (3), (3), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1)