Coagulation Factor II (thrombin) Receptor (F2R) antibody

Antigen: Coagulation Factor II (thrombin) Receptor (F2R)

Synonyms: TR, HTR, CF2R, PAR1, PAR-1, Par1, TRGPC, MGC93622

Clonality: Monoclonal (B825M)

Host: Mouse

Reactivity: Please enquire

Conjugate: Un-conjugated

Application: Flow Cytometry (FACS), Immunoprecipitation (IP), Western Blotting (WB)

Catalog no.: ABIN236474

Additional Information

Alternative name: Thrombin Receptor

Immunogen: N-terminal peptide of thrombin receptor residues 51-64 (KYEPFWEDEEKNES)

Format: Lyophilized

Isotype: IgG1

Clone: B825M

Description: MAb to Thrombin Receptor Monoclonal Antibody to Thrombin Receptor

Specificity: Recognizes an epitope on the receptor, distant from the N-terminal thrombin cleavage site (WEDEE). It reacts with both intact and cleaved receptors.

Application Details

Application Notes: Suitable for use in Western blot, immunoprecipitation and Flow cytometry (2ug/5x105 cells or 2ug/100ul whole blood). Each laboratory should determine an optimum working titer for use in its particular application. Other applications have not been tested but use in such assays should not necessarily be excluded.

Concentration: 0.2mg/ml (prior to lyophilization)

Purification: Source: Ascites

Buffer: Lyophilized from PBS containing 1mg/ml BSA.

Storage: Store lyophilized product at 2-8°C. After reconstitution, aliquot and store at -20°C. Avoid multiple freeze/thaw cycles. The addition of 0.1% (w/v) sodium azide is recommended for storage (up to one month) at 2-8°C.Reconstitute with 1ml distilled water.

Research Area: Chromatin and Nuclear Signaling, Cell Cycle, Angiogenesis, Coagulation

Restrictions: For Research Use only

Publications

Brass, Vassallo, Belmonte et al.: "Structure and function of the human platelet thrombin receptor. Studies using monoclonal antibodies directed against a defined domain within the receptor N terminus." in: The Journal of biological chemistry, Vol. 267, Issue 20, pp. 13795-8, 1992 (PubMed).

Vu, Hung, Wheaton et al.: "Molecular cloning of a functional thrombin receptor reveals a novel proteolytic mechanism of receptor activation." in: Cell, Vol. 64, Issue 6, pp. 1057-68, 1991 (PubMed).

Woolkalis, DeMelfi, Blanchard et al.: "Regulation of thrombin receptors on human umbilical vein endothelial cells." in: The Journal of biological chemistry, Vol. 270, Issue 17, pp. 9868-75, 1995 (PubMed).

Molino, Blanchard, Belmonte et al.: "Proteolysis of the human platelet and endothelial cell thrombin receptor by neutrophil-derived cathepsin G." in: The Journal of biological chemistry, Vol. 270, Issue 19, pp. 11168-75, 1995 (PubMed).

Hoxie, Ahuja, Belmonte et al.: "Internalization and recycling of activated thrombin receptors." in: The Journal of biological chemistry, Vol. 268, Issue 18, pp. 13756-63, 1993 (PubMed).