U1 Small Nuclear Ribonucleoprotein (SnRNP) A/B antibody

Antigen: U1 Small Nuclear Ribonucleoprotein (SnRNP) A/B

Clonality: Monoclonal (9A9)

Host: Mouse

Reactivity: Human

Application: Immunofluorescence (IF), Immunoprecipitation (IP), Western Blotting (WB)

Catalog no.: ABIN236620

Additional Information

Immunogen: Recombinant beta-galactosidase U2B? fusion protein.

Format: Supernatant, Lyophilized

Isotype: IgG1  (Matching secondary antibodies)

Clone: 9A9

Description: MAb to U1 snRNP A/B Monoclonal Antibody to U1 small nuclear Ribonucleoprotein (snRNP) A/B Cell culture

Specificity: Reacts primarily with the human U1A protein and has minor cross-reactivity with the U2B? protein, contained in the U1 and U2 small nuclear ribonucleoprotein (snRNP) complexes.

Application Details

Application Notes: Immunoprecipitation Immunofluorescence microscopy (1:5?1:10) Western blot (1:5?1:10) Each laboratory should determine an optimum working titer for use in its particular application. Other applications have not been tested but use in such assays should not necessarily be excluded.

Concentration: Not determined

Purification: Not applicable. Source: Cell culture

Buffer: Lyophilized from PBS containing 1% BSA. Preservative: 0.1% Sodium azide

Storage: Store lyophilized product at 2-8°C. After reconstitution, aliquot and store at -20°C. Avoid multiple freeze/thaw cycles.Reconstitute in 1ml distilled water

Restrictions: For Research Use only

Publications

Publications: Habets, Hoet, De Jong et al.: "Mapping of B cell epitopes on small nuclear ribonucleoproteins that react with human autoantibodies as well as with experimentally-induced mouse monoclonal antibodies." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 143, Issue 8, pp. 2560-6, 1989 (PubMed).