Ubiquitin (AA 50-65) antibody

Antigen: Ubiquitin

Synonyms: MGC8385, FLJ25987, Ubc, Ubb2, Uba52, Rps27a, AL033289, CG11624, DmelCG11624, DmUb, Ub, ubi, Ubi-p, ubi-p63E, Ubi-P63e, ubi63E, Ubi63E, Ubi63p, Ubip, Ubiq, Ubp, UBC, CG18282, CG32744, CR32744, DmelCG32744, ubiquitin, si:dz180g5.5, si:busm1-180g5.5, UBB, Ubiquitin, CG11700, ESTS:143G11T, ESTS:199A6T, anon-sts41, poly-ub, DmelCR11700, LOC100194618, UBIQ, LOC100282068

Binding Site: AA 50-65

Clonality: Monoclonal (3-39)

Host: Mouse

Reactivity: Please enquire

Conjugate: Un-conjugated

Application: ELISA, Immunohistochemistry (IHC), Immunoprecipitation (IP), Western Blotting (WB)

Catalog no.: ABIN236621

Additional Information

Isotype: IgG1, kappa

Clone: 3-39

Description: MAb to Ubiquitin (a.a. 50-65) Monoclonal Antibody to Ubiquitin (amino acids 50-65)

Specificity: Ubiquitin proteins. Residues 50-65 of ubiquitin. Reactive only to ubiquitinated protein, not to free ubiquitin. Highly reactive on formalin-fixed tissues. Can be used on native or detergent extracted tissues and the epitope is resistant to proteolytic enzyme treatment.

Synonyms: MGC8385, FLJ25987, Ubc, Ubb2, Uba52, Rps27a, AL033289, CG11624, DmelCG11624, DmUb, Ub, ubi, Ubi-p, ubi-p63E, Ubi-P63e, ubi63E, Ubi63E, Ubi63p, Ubip, Ubiq, Ubp, UBC, CG18282, CG32744, CR32744, DmelCG32744, ubiquitin, si:dz180g5.5, si:busm1-180g5.5, UBB, Ubiquitin, CG11700, ESTS:143G11T, ESTS:199A6T, anon-sts41, poly-ub, DmelCR11700, LOC100194618, UBIQ, LOC100282068

Application Details

Application Notes: ELISA (1:10,000 ? 1:100,000) Western blot (1:1,000 ? 1:100,000) Immunoprecipitation (1:10 ? 1:100) Immunohistochemistry (1:100 ? 1:10,000) Each laboratory should determine an optimum working titer for use in its particular application. Other applications have not been tested but use in such assays should not necessarily be excluded.

Concentration: ~2mg/ml (Bradford & ELISA assays)

Purification: Purified. Product is 0.2µm filtered.

Buffer: Not applicable.

Storage: Best stored undiluted at -20°C to -70°C. Diluted antibody can be stored frozen or refrigerated in the presence of 0.1% sodium azide or other anti-bacterial agent. Avoid multiple freeze/thaw cycles.

Restrictions: For Research Use only

Publications

Wang, Iqbal, Bucht et al.: "Alzheimer's disease: paired helical filament immunoreactivity in cerebrospinal fluid." in: Acta neuropathologica, Vol. 82, Issue 1, pp. 6-12, 1991 (PubMed).

Grundke-Iqbal, Wang, Iqbal et al.: "Alzheimer paired helical filaments: identification of polypeptides with monoclonal antibodies." in: Acta neuropathologica, Vol. 68, Issue 4, pp. 279-83, 1986 (PubMed).

Galloway, Grundke-Iqbal, Iqbal et al.: "Lewy bodies contain epitopes both shared and distinct from Alzheimer neurofibrillary tangles." in: Journal of neuropathology and experimental neurology, Vol. 47, Issue 6, pp. 654-63, 1988 (PubMed).

Perry, Mulvihill, Fried et al.: "Immunochemical properties of ubiquitin conjugates in the paired helical filaments of Alzheimer disease." in: Journal of neurochemistry, Vol. 52, Issue 5, pp. 1523-8, 1989 (PubMed).

Grundke-Iqbal, Iqbal, Quinlan et al.: "Microtubule-associated protein tau. A component of Alzheimer paired helical filaments." in: The Journal of biological chemistry, Vol. 261, Issue 13, pp. 6084-9, 1986 (PubMed).

Wang, Grundke-Iqbal, Kascsak et al.: "Alzheimer neurofibrillary tangles: monoclonal antibodies to inherent antigen(s)." in: Acta neuropathologica, Vol. 62, Issue 4, pp. 268-75, 1984 (PubMed).