Ubiquitin (AA 64-76) antibody

Antigen: Ubiquitin

Synonyms: MGC8385, FLJ25987, Ubc, Ubb2, Uba52, Rps27a, AL033289, CG11624, DmelCG11624, DmUb, Ub, ubi, Ubi-p, ubi-p63E, Ubi-P63e, ubi63E, Ubi63E, Ubi63p, Ubip, Ubiq, Ubp, UBC, CG18282, CG32744, CR32744, DmelCG32744, ubiquitin, si:dz180g5.5, si:busm1-180g5.5, UBB, Ubiquitin, CG11700, ESTS:143G11T, ESTS:199A6T, anon-sts41, poly-ub, DmelCR11700, LOC100194618, UBIQ, LOC100282068

Binding Site: AA 64-76

Clonality: Monoclonal (5-25)

Host: Mouse

Reactivity: Please enquire

Conjugate: Un-conjugated

Application: ELISA, Immunohistochemistry (IHC), Immunoprecipitation (IP), Western Blotting (WB)

Catalog no.: ABIN236622

Additional Information

Isotype: IgG1, kappa

Clone: 5-25

Description: MAb to Ubiquitin (a.a. 64-76) Monoclonal Antibody to Ubiquitin (amino acids 64-76)

Specificity: Ubiquitin, Ubiquitinated proteins. Residues 64-76 of Ubiquitin. Reactive to free and bound Ubiquitin, to native or detergent extracted tissues. Formalin fixation eliminates immunoreactivity.

Synonyms: MGC8385, FLJ25987, Ubc, Ubb2, Uba52, Rps27a, AL033289, CG11624, DmelCG11624, DmUb, Ub, ubi, Ubi-p, ubi-p63E, Ubi-P63e, ubi63E, Ubi63E, Ubi63p, Ubip, Ubiq, Ubp, UBC, CG18282, CG32744, CR32744, DmelCG32744, ubiquitin, si:dz180g5.5, si:busm1-180g5.5, UBB, Ubiquitin, CG11700, ESTS:143G11T, ESTS:199A6T, anon-sts41, poly-ub, DmelCR11700, LOC100194618, UBIQ, LOC100282068

Application Details

Application Notes: ELISA (1:10,000 1:100,000) Western blot (1:1,000 ? 1:100,000) Immunoprecipitation (1:10 ? 1:100) Immunohistochemistry on unfixed tissues (1:100 ? 1:10,000) Each laboratory should determine an optimum working titer for use in its particular application. Other applications have not been tested but use in such assays should not necessarily be excluded.

Concentration: ~2mg/ml (Bradford & ELISA assays)

Purification: Purified. Product is 0.2µm filtered.

Buffer: Not applicable.

Storage: Store at -20°C to -70°C. Avoid multiple freeze/thaw cycles.

Restrictions: For Research Use only

Publications

Perry, Mulvihill, Fried et al.: "Immunochemical properties of ubiquitin conjugates in the paired helical filaments of Alzheimer disease." in: Journal of neurochemistry, Vol. 52, Issue 5, pp. 1523-8, 1989 (PubMed).

Grundke-Iqbal, Iqbal, Quinlan et al.: "Microtubule-associated protein tau. A component of Alzheimer paired helical filaments." in: The Journal of biological chemistry, Vol. 261, Issue 13, pp. 6084-9, 1986 (PubMed).

Wang, Grundke-Iqbal, Kascsak et al.: "Alzheimer neurofibrillary tangles: monoclonal antibodies to inherent antigen(s)." in: Acta neuropathologica, Vol. 62, Issue 4, pp. 268-75, 1984 (PubMed).

Ping, Zhou, Sun et al.: "[A new genus of oral bacteria in human]" in: Wei sheng wu xue bao = Acta microbiologica Sinica, Vol. 38, Issue 2, pp. 146-51, 2003 (PubMed).