Apolipoprotein E (APOE) antibody

Antigen: Apolipoprotein E (APOE)

Synonyms: AD2, LPG, LDLCQ5, MGC1571, AI255918, APOEA, APOE, ad2, apoprotein, MGC110064, im:7036787, wu:fb69a05, zgc:110064, apoe

Clonality: Polyclonal

Host: Rabbit

Reactivity: Mouse (Murine)

Conjugate: Un-conjugated

Application: Immunodiffusion (ID), Immunoelectrophoresis (IEP), Radial Immunodiffusion (RID), Western Blotting (WB)

Catalog no.: ABIN236817

Additional Information

Alternative name: Apolipoprotein E (Apo E)

Immunogen: Purified Mouse Apolipoprotein E from pooled mouse plasma high and very low density lipoproteins

Format: Liquid

Description: Rabbit A’ Mouse Apo E Rabbit Antibody to Mouse Apolipoprotein E (Apo E)

Specificity: Species specific: extremely low recognition of human Apo E. No data available regarding rat activity.

Application Details

Application Notes: Suitable for use in Radial immunodiffusion, immuno double-diffusion, rocket IEP and Western blot. Starting dilution for Western blot: 1:500 - 1:1,000. Each laboratory should determine an optimum working titer for use in its particular application. Other applications have not been tested but use in such assays should not necessarily be excluded.

Concentration: Not determined Titer: 1:40 (RID)

Purification: Neat antiserumHeat inactivated Product is 0.22um filtered.

Buffer: Not applicable. Preservative: 0.05% Sodium azide

Storage: Upon receipt, aliquot and store (up to 1 year) at -70°C. Avoid multiple freeze/thaw cycles. : This product contains sodium azide, which has been classified as Xn (Harmful), in European Directive 67/548/EEC in the concentration range of 0.1-1.0%. When disposing of this reagent through lead or copper plumbing, flush with copious volumes of water to prevent azide build-up in drains.

Restrictions: For Research Use only

Publications

Rees, Sloane, Jessup et al.: "Apolipoprotein A-I stimulates secretion of apolipoprotein E by foam cell macrophages." in: The Journal of biological chemistry, Vol. 274, Issue 39, pp. 27925-33, 1999 (PubMed).

Hayhurst, Lee, Lambert et al.: "Hepatocyte nuclear factor 4alpha (nuclear receptor 2A1) is essential for maintenance of hepatic gene expression and lipid homeostasis." in: Molecular and cellular biology, Vol. 21, Issue 4, pp. 1393-403, 2001 (PubMed).

Vaisman, Lambert, Amar et al.: "ABCA1 overexpression leads to hyperalphalipoproteinemia and increased biliary cholesterol excretion in transgenic mice." in: The Journal of clinical investigation, Vol. 108, Issue 2, pp. 303-9, 2001 (PubMed).