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Caspase 9, Apoptosis-Related Cysteine Peptidase (CASP9) (Middle Region 2) antibody

Antigen

Caspase 9, Apoptosis-Related Cysteine Peptidase (CASP9)
Synonyms MCH6, APAF3, APAF-3, ICE-LAP6, CASPASE-9c, Mch6, AI115399, AW493809, Caspase-9, CASP9, LOC100101592
Binding Site
Alternatives

Middle Region 2
Clonality Polyclonal
Host
Alternatives

Rabbit
Reactivity
Conjugate
Alternatives Un-conjugated
Application
Alternatives Western Blotting (WB)
4 references available
Catalog no. ABIN236956
Quantity 100ug  (0.5mg/ml)
Price 468.60 $   Plus shipping costs $45.00
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Additional Information
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Alternative name Caspase-9
Immunogen Peptide corresponding to amino acids 299-318 of human caspase-9
Format Liquid
Description Rabbit anti Caspase-9 (I20) (Intermed. Domain 2) Rabbit Antibody to Caspase-9 (I20), Intermediate Domain 2
Specificity Reacts with human caspase-9 and has no cross-reactivity with other members of the caspase family. Also known as Mch6/Apaf (IN2). Caspase-9 play a central role in cell death induced by a wide variety of apoptosis activators including TNF-alpha, TRAIL, anti-CD-95, FADD and TRADD. (4) Caspase-9 is expressed in a variety of human tissues. (1, 2)

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Application Notes Suitable for use in immunohistochemistry (2ug/ml) and Western blot (1:1,000-1:2,000). Each laboratory should determine an optimum working titer for use in its particular application. Other applications have not been tested but use in such assays should not necessarily be excluded.
Concentration 0.5mg/ml
Purification Immunoaffinity chromatography
Buffer PBS. Preservative: 0.02% Sodium azide
Storage Store (up to one year) at 2-8°C. : This product contains sodium azide, which has been classified as Xn (Harmful), in European Directive 67/548/EEC in the concentration range of 0.1-1.0%. When disposing of this reagent through lead or copper plumbing, flush with copious volumes of water to prevent azide build-up in drains.
Restrictions For Research Use only

Publications
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Duan, Orth, Chinnaiyan et al.: "ICE-LAP6, a novel member of the ICE/Ced-3 gene family, is activated by the cytotoxic T cell protease granzyme B." in: The Journal of biological chemistry, Vol. 271, Issue 28, pp. 16720-4, 1996 (PubMed).

Srinivasula, Fernandes-Alnemri, Zangrilli et al.: "The Ced-3/interleukin 1beta converting enzyme-like homolog Mch6 and the lamin-cleaving enzyme Mch2alpha are substrates for the apoptotic mediator CPP32." in: The Journal of biological chemistry, Vol. 271, Issue 43, pp. 27099-106, 1996 (PubMed).

Li, Nijhawan, Budihardjo et al.: "Cytochrome c and dATP-dependent formation of Apaf-1/caspase-9 complex initiates an apoptotic protease cascade." in: Cell, Vol. 91, Issue 4, pp. 479-89, 1997 (PubMed).

Pan, ORourke, Dixit: "Caspase-9, Bcl-XL, and Apaf-1 form a ternary complex." in: The Journal of biological chemistry, Vol. 273, Issue 10, pp. 5841-5, 1998 (PubMed).

Alternatives
Application Details Publications back to top

Alternatives for antigen "Caspase 9, Apoptosis-Related Cysteine Peptidase (CASP9)", type "Antibodies"
Hosts Rabbit (245), Mouse (16), Goat (1)
Reactivities Human (245), Rat (Rattus) (111), Mouse (Murine) (90), Dog (Canine) (24), Cow (Bovine) (23), Hamster (4), Monkey (4), Pig (Porcine) (3), Rabbit (3), Gerbil (2), Cat (Feline) (1), Chicken (1), Sheep (Ovine) (1)
Applications Western Blotting (WB) (179), Immunofluorescence (IF) (103), ELISA (74), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (61), Immunoprecipitation (IP) (41), Immunohistochemistry (IHC) (27), Immunocytochemistry (ICC) (20), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (15), Immunohistochemistry (Frozen Sections) (IHC (fro)) (7), Immunoelectron Microscopy (IEM) (6), Immunohistochemistry (Fixed) (IHC (fx)) (5), Flow Cytometry (FACS) (3)
Conjugates Biotin (8), Alexa Fluor 350 (5), Alexa Fluor 488 (5), Alexa Fluor 555 (5), Alexa Fluor 647 (5), Cy3 (5), Cy5 (5), Cy5.5 (5), Cy7 (5), FITC (5), Gold (5), HRP (5), PE (5), PE,Cy3 (5), PE,Cy5 (5), PE,Cy5.5 (5), PE,Cy7 (5)
Epitopes N-Term (26), C-Term (25), pSer196 (22), pThr125 (19), pTyr153 (19), Internal Region (12), AA 299-318 (7), AA 41-56 (5), active (4), pThr125,Internal Region (3), AA 112-130 (2), AA 188-206 (2), AA 301-305 (2), Asp315 (2), Asp353 (2), Center (2), Pro-form (2), Ser196 (2), AA 1 (1), AA 1-134 (1), AA 287-306 (1), AA 345-359 (1), Cleavage Site (1), Cleaved-Asp315 (1), Cleaved-Asp330 (1), Cleaved-Asp353 (1), Middle Region 1 (1), Middle Region 2 (1), N-Term,Asp330,active (1), cleaved (1), pSer144 (1)