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Antibodies
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anti-Collagen, Type I (COL1) antibody
Collagen, Type I (COL1) antibody
| Antigen |
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| Clonality |
Polyclonal |
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Host
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Reactivity
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Human (70), Rat (Rattus) (30), Mouse (Murine) (27), Cow (Bovine) (25), Dog (Canine) (19), Horse (Equine) (18), Sheep (Ovine) (16), Rabbit (15), Chicken (8), Fish (3), Amphibian (2), Bird (Avian) (2), Mammalian (2), Pig (Porcine) (2)
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Application
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Alternatives Dot Blot (Dot), ELISA, Immunocytochemistry (ICC), Immunohistochemistry (Frozen Sections) (IHC (fro))
ELISA (77), Immunohistochemistry (Frozen Sections) (IHC (fro)) (56), Immunofluorescence (IF) (53), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (33), Western Blotting (WB) (24), Flow Cytometry (FACS) (18), Radioimmunoassay (RIA) (18), Immunocytochemistry (ICC) (10), Immunoprecipitation (IP) (9), Immunohistochemistry (IHC) (4), Dot Blot (Dot) (3), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (2), Immunoelectron Microscopy (IEM) (1), Solid Phase Radioimmunoassay (SP-RIA) (1)
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| Catalog no. |
ABIN237014 |
| Quantity |
0.2mg (0.4mg/ml) (Variants) |
| Price |
472.67 $ Plus shipping costs $35.00
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| Shipping to |
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| Availability |
Ships within 5 to 7 Business Days |
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Alternative name
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Human Collagen Type I
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Immunogen
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Human Type I collagen
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Format
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Affinity-purified, Liquid
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Description
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Goat anti Collagen Type I Goat Antibody to Human Collagen Type I
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Specificity
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Reacts with conformational determinants on human type I collagen as demonstrated by ELISA. May react with type I collagen from other species. Exhibits <10% cross reactivity with collagen types II, III, IV, V and VI. Has not been tested for reactivity with other extracellular matrix proteins (e.g. laminin, fibronectin).
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Application Notes
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Dot- and slot-immunoblotting (non-denaturing) Immunohistochemistry (frozen sections)/Immunocytochemistry: 1:10?1:20. Enzyme linked immunosorbent assay (ELISA) Each laboratory should determine an optimum working titer for use in its particular application. Other applications have not been tested but use in such assays should not necessarily be excluded.
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Concentration
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0.4mg/ml
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Purification
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Affinity chromatography on human Type I collagen covalently linked to agarose. Cross absorption on collagen types II, III, IV, V and VI covalently linked to agarose.
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Buffer
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100mM Borate buffered saline, pH 8.2.
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Storage
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Store at 2-8°C.
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Restrictions
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For Research Use only
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Alternatives for antigen "Collagen, Type I (COL1)", type "Antibodies"
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Hosts
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Rabbit (71), Goat (16), Mouse (16), Bovine (2), Rat (2), Human (1)
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Reactivities
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Human (70), Rat (Rattus) (30), Mouse (Murine) (27), Cow (Bovine) (25), Dog (Canine) (19), Horse (Equine) (18), Sheep (Ovine) (16), Rabbit (15), Chicken (8), Fish (3), Amphibian (2), Bird (Avian) (2), Mammalian (2), Pig (Porcine) (2)
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Applications
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ELISA (77), Immunohistochemistry (Frozen Sections) (IHC (fro)) (56), Immunofluorescence (IF) (53), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (33), Western Blotting (WB) (24), Flow Cytometry (FACS) (18), Radioimmunoassay (RIA) (18), Immunocytochemistry (ICC) (10), Immunoprecipitation (IP) (9), Immunohistochemistry (IHC) (4), Dot Blot (Dot) (3), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (2), Immunoelectron Microscopy (IEM) (1), Solid Phase Radioimmunoassay (SP-RIA) (1)
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Conjugates
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Biotin (11), None (5), Alexa Flour 488 (1), Alexa Fluor 350 (1), Alexa Fluor 488 (1), Alexa Fluor 555 (1), Alexa Fluor 647 (1), Cy3 (1), Cy5 (1), Cy5.5 (1), Cy7 (1), FITC (1), Gold (1), HRP (1), PE (1), PE,Cy3 (1), PE,Cy5 (1), PE,Cy5.5 (1), PE,Cy7 (1), RBITC (1)
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