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Antibodies
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anti-Collagen, Type I (COL1) antibody (Biotin)
Collagen, Type I (COL1) antibody (Biotin)
| Antigen |
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| Clonality |
Polyclonal |
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Host
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Reactivity
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Human (70), Rat (Rattus) (30), Mouse (Murine) (27), Cow (Bovine) (25), Dog (Canine) (19), Horse (Equine) (18), Sheep (Ovine) (16), Rabbit (15), Chicken (8), Fish (3), Amphibian (2), Bird (Avian) (2), Mammalian (2), Pig (Porcine) (2)
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Conjugate
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Biotin (10), None (5), Alexa Flour 488 (1), Alexa Fluor 350 (1), Alexa Fluor 488 (1), Alexa Fluor 555 (1), Alexa Fluor 647 (1), Cy3 (1), Cy5 (1), Cy5.5 (1), Cy7 (1), FITC (1), Gold (1), HRP (1), PE (1), PE,Cy3 (1), PE,Cy5 (1), PE,Cy5.5 (1), PE,Cy7 (1), RBITC (1)
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Application
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Alternatives Dot Blot (Dot), ELISA, Immunocytochemistry (ICC), Immunohistochemistry (Frozen Sections) (IHC (fro))
ELISA (77), Immunohistochemistry (Frozen Sections) (IHC (fro)) (56), Immunofluorescence (IF) (53), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (33), Western Blotting (WB) (24), Flow Cytometry (FACS) (18), Radioimmunoassay (RIA) (18), Immunocytochemistry (ICC) (10), Immunoprecipitation (IP) (9), Immunohistochemistry (IHC) (4), Dot Blot (Dot) (3), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (2), Immunoelectron Microscopy (IEM) (1), Solid Phase Radioimmunoassay (SP-RIA) (1)
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| Catalog no. |
ABIN237015 |
| Quantity |
0.2mg (0.4mg/ml) (Variants) |
| Price |
628.67 $ Plus shipping costs $35.00
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| Shipping to |
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| Availability |
Ships within 5 to 7 Business Days |
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Alternative name
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Human Collagen Type I
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Immunogen
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Human type I collagen
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Format
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Liquid
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Description
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Goat A’ Human Collagen Type I Goat Antibody to Human Collagen Type I Biotin conjugated
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Specificity
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Reacts with conformational determinants on human type I collagen as demonstrated by ELISA. May react with type I collagen from other species. Exhibits <10% cross reactivity with collagen types II, III, IV, V, and VI. Has not been tested for reactivity with other extracellular matrix proteins (e.g. laminin, fibronectin).
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Application Notes
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Dot-and slot-immunoblotting Immunocytochemistry: 1:20?1:40 Immunohistochemistry (frozen sections only), 1:20?1:40 ELISA 1:1,000?1:4,000 Each laboratory should determine an optimum working titer for use in its particular application. Other applications have not been tested but use in such assays should not necessarily be excluded.
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Concentration
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0.4mg/ml
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Purification
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Affinity chromatography on human type I collagen covalently linked to agarose. Cross absorption on collagen types II, III, IV, V and VI covalently linked to agarose.
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Buffer
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PBS, pH 7.4. Preservative: 0.1% Sodium azide
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Storage
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Store (protected from prolonged exposure to light) at 2-8°C. : This product contains sodium azide, which has been classified as Xn (Harmful), in European Directive 67/548/EEC in the concentration range of 0.1-1.0%. When disposing of this reagent through lead or copper plumbing, flush with copious volumes of water to prevent azide build-up in drains.
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Restrictions
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For Research Use only
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Alternatives for antigen "Collagen, Type I (COL1)", type "Antibodies"
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Hosts
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Rabbit (71), Goat (16), Mouse (16), Bovine (2), Rat (2), Human (1)
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Reactivities
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Human (70), Rat (Rattus) (30), Mouse (Murine) (27), Cow (Bovine) (25), Dog (Canine) (19), Horse (Equine) (18), Sheep (Ovine) (16), Rabbit (15), Chicken (8), Fish (3), Amphibian (2), Bird (Avian) (2), Mammalian (2), Pig (Porcine) (2)
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Applications
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ELISA (77), Immunohistochemistry (Frozen Sections) (IHC (fro)) (56), Immunofluorescence (IF) (53), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (33), Western Blotting (WB) (24), Flow Cytometry (FACS) (18), Radioimmunoassay (RIA) (18), Immunocytochemistry (ICC) (10), Immunoprecipitation (IP) (9), Immunohistochemistry (IHC) (4), Dot Blot (Dot) (3), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (2), Immunoelectron Microscopy (IEM) (1), Solid Phase Radioimmunoassay (SP-RIA) (1)
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Conjugates
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Biotin (10), None (5), Alexa Flour 488 (1), Alexa Fluor 350 (1), Alexa Fluor 488 (1), Alexa Fluor 555 (1), Alexa Fluor 647 (1), Cy3 (1), Cy5 (1), Cy5.5 (1), Cy7 (1), FITC (1), Gold (1), HRP (1), PE (1), PE,Cy3 (1), PE,Cy5 (1), PE,Cy5.5 (1), PE,Cy7 (1), RBITC (1)
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