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Antibodies
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anti-Collagen, Type I (COL1) antibody
Collagen, Type I (COL1) antibody
| Antigen |
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| Clonality |
Polyclonal |
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Host
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Reactivity
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Human (71), Rat (Rattus) (30), Mouse (Murine) (27), Cow (Bovine) (25), Dog (Canine) (19), Horse (Equine) (18), Sheep (Ovine) (16), Rabbit (15), Chicken (8), Amphibian (2), Bird (Avian) (2), Fish (2), Mammalian (2), Pig (Porcine) (2)
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Application
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 Alternatives ELISA, Immunofluorescence (IF), Solid Phase Radioimmunoassay (SP-RIA)
ELISA (77), Immunohistochemistry (Frozen Sections) (IHC (fro)) (57), Immunofluorescence (IF) (52), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (33), Western Blotting (WB) (24), Flow Cytometry (FACS) (18), Radioimmunoassay (RIA) (18), Immunocytochemistry (ICC) (11), Immunoprecipitation (IP) (9), Dot Blot (Dot) (4), Immunohistochemistry (IHC) (4), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (2), Immunoelectron Microscopy (IEM) (1)
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| Catalog no. |
ABIN237021 |
| Quantity |
0.5ml (Not applicable) (Variants) |
| Price |
788.67 $ Plus shipping costs $35.00
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| Shipping to |
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| Availability |
Ships within 5 to 7 Business Days |
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Alternative name
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Fish Collagen Type I
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Immunogen
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Collagen Type I extracted and purified from salmon fish skin
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Format
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Purified, Lyophilized
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Description
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Rabbit A’ Fish Collagen Type I Rabbit Antibody to Fish Collagen Type I
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Specificity
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Cross-reactivity: (OD at a 1:100 ELISA dilution) Salmon Fish Collagen Type I >1.0, Sole and Tuna fish Collagen Type I under0.4, Goldfish Collagen Type I under0.2*, Mammalian, avian Collagen Type I under0.2* (*OD under 0.2 means negative reactivity)
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Application Notes
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Suitable for use in IFA, (SP)RIA, ELISA (tested). For immunostaining of extra or intracellular components in light microscopy and the quantitation of Salmon Type I collagen. In indirect IFA with fluorescein anti-rabbit IgG conjugate, use diluted at 1:40 on frozen salmon tissues (skin, liver). For ELISA, use diluted at 1:10-1:200. Each laboratory should determine an optimum working titer for use in its particular application. Other applications have not been tested but use in such assays should not necessarily be excluded.
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Concentration
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Not applicable
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Purification
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Ion exchange chromatography
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Buffer
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Not applicable.
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Storage
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Lyophilized: Short-term (up to 24 months) store at 2-8°C. Long term store at -20°C. Reconstituted: Aliquot and store (up to 6 months) at -20°C. Avoid multiple freeze/thaw cycles.Reconstitute with 0.5ml deionized water.
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Restrictions
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For Research Use only
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Alternatives for antigen "Collagen, Type I (COL1)", type "Antibodies"
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Hosts
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Rabbit (70), Goat (17), Mouse (16), Bovine (2), Rat (2), Human (1)
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Reactivities
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Human (71), Rat (Rattus) (30), Mouse (Murine) (27), Cow (Bovine) (25), Dog (Canine) (19), Horse (Equine) (18), Sheep (Ovine) (16), Rabbit (15), Chicken (8), Amphibian (2), Bird (Avian) (2), Fish (2), Mammalian (2), Pig (Porcine) (2)
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Applications
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ELISA (77), Immunohistochemistry (Frozen Sections) (IHC (fro)) (57), Immunofluorescence (IF) (52), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (33), Western Blotting (WB) (24), Flow Cytometry (FACS) (18), Radioimmunoassay (RIA) (18), Immunocytochemistry (ICC) (11), Immunoprecipitation (IP) (9), Dot Blot (Dot) (4), Immunohistochemistry (IHC) (4), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (2), Immunoelectron Microscopy (IEM) (1)
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Conjugates
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Biotin (11), None (5), Alexa Flour 488 (1), Alexa Fluor 350 (1), Alexa Fluor 488 (1), Alexa Fluor 555 (1), Alexa Fluor 647 (1), Cy3 (1), Cy5 (1), Cy5.5 (1), Cy7 (1), FITC (1), Gold (1), HRP (1), PE (1), PE,Cy3 (1), PE,Cy5 (1), PE,Cy5.5 (1), PE,Cy7 (1), RBITC (1)
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