Collagen, Type V (COL5) antibody

Antigen: Collagen, Type V (COL5)

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Application: ELISA, Immunofluorescence (IF), Solid Phase Radioimmunoassay (SP-RIA)

Catalog no.: ABIN237045

Additional Information

Alternative name: Human Collagen Type V

Immunogen: Collagen Type V extracted and purified from human placenta.

Format: Purified, Lyophilized

Description: Rabbit A’ Human Collagen Type V Rabbit Antibody to Human Collagen Type V

Specificity: Cross-reactivity (RIA%) Human collagen type V 100% Human collagen type I <0.1% Human collagen type III <0.1% Human collagen type IV <0.1% Human Fibronectin <0.1% Mouse Laminin <0.1%

Application Details

Application Notes: IFA, (SP)RIA and ELISA. For immunostaining of extra or intracellular components in light microscopy. For the quantitation of Collagen Type V in biological fluids or in culture supernatants. In indirect IFA with fluorescein anti-rabbit IgG conjugate, use diluted at >1:40 on frozen human skin, liver and placenta tissues. In ELISA, use diluted at >1:100-1:200. Each laboratory should determine an optimum working titer for use in its particular application. Other applications have not been tested but use in such assays should not necessarily be excluded.

Concentration: Not determined Titer: 1:100 (RIA)

Purification: Ion exchange chromatography (DEAE-Trisacryl)

Buffer: Not applicable.

Storage: Lyophilized: Store (up to 24 months) at 2-8°C. Long term store at -20°C. Reconstituted: Aliquot and store (up to 6 months) at -20°C. Avoid multiple freeze/thaw cycles.Reconstitute with 0.5ml deionized water.

Restrictions: For Research Use only