Cullin 1 (CUL1) (C-Term), (AA 766-776) antibody

Details for Product No. ABIN237093
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Antigen
Epitope
C-Term, AA 766-776
(34), (8), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity
Human
(88), (40), (24), (13), (12), (2), (2), (2), (1), (1), (1), (1)
Host
Rabbit
(82), (6), (1)
Clonality
Polyclonal
Conjugate
Un-conjugated
(2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application
ELISA, Immunoprecipitation (IP), Western Blotting (WB)
(73), (27), (27), (27), (19), (17), (10), (7), (6), (3), (2), (1), (1), (1)
Pubmed 3 references available
Quantity 100 μL
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Catalog No. ABIN237093
702.53 $
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Immunogen A synthetic peptide corresponding to AA 766-776 of Human Cul1 (C-terminus) coupled to KLH. Sequence: DGEKDTYSYLA
Specificity Reacts with human Cullin 1.
Characteristics Rabbit anti Cul1 (AA 766-776), Rabbit antibody to Cullin 1 (Cul1), C-terminal (AA 766-776)
Purification Neat antiserum delipidation and defibrination.
Sterility Sterile filtered
Application Notes Suitable for use in ELISA (1:2.000 - 1:10.000) and Western blot (1:500 - 1:1.000). The antibody immunoprecipitates in vitro translated protein and protein from cell lysates (using HeLa or NIH-3T3). NOTE: Do not Immunoprecipitate in the presence of NP-40, but rather use 0.1 % SDS. An 89.6 kDa band corresponding to human Cul1 is detected. Each laboratory should determine an optimum working titer for use in its particular application. Other applications have not been tested but use in such assays should not necessarily be excluded.
Restrictions For Research Use only
Format Liquid
Concentration Total Protein: 85 mg/mL (Refractometry)
Buffer 0.01 % (w/v) Sodium Azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid multiple freeze/thaw cycles. Prepare working dilution only prior to immediate use.
Centrifuge product if not completely clear after standing at room temperature.
Prepare working dilution only prior to immediate use.
Storage -20 °C
Storage Comment Upon receipt, aliquot and store at -20 °C or below.
General Kipreos, Lander, Wing et al.: "cul-1 is required for cell cycle exit in C. elegans and identifies a novel gene family." in: Cell, Vol. 85, Issue 6, pp. 829-39, 1996 (PubMed).

Mathias, Johnson, Winey et al.: "Cdc53p acts in concert with Cdc4p and Cdc34p to control the G1-to-S-phase transition and identifies a conserved family of proteins." in: Molecular and cellular biology, Vol. 16, Issue 12, pp. 6634-43, 1997 (PubMed).

Le Meignen, Mossler, Mates et al.: "[Management of immune thrombocytopenic purpura: A prospective study of 147 children from the regional network RH" in: Archives de pediatrie : organe officiel de la Societe francaise de pediatrie, 2008 (PubMed).

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