Cullin 1 (CUL1) (C-Term AA 766-776) antibody

Antigen: Cullin 1 (CUL1)

Synonyms: LOC100282292, CUL1, Cullin-1, DKFZp459I053, MGC149834, MGC149835, Cul1, zgc:55747, zgc:77500, wu:fc13a10

Binding Site: C-Term AA 766-776

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Conjugate: Un-conjugated

Application: ELISA, Immunoprecipitation (IP), Western Blotting (WB)

Catalog no.: ABIN237093

Additional Information

Immunogen: A synthetic peptide corresponding to amino acids 766-776 of Human Cul1 (C-terminus) coupled to KLH. Sequence: DGEKDTYSYLA

Format: Liquid

Description: Rabbit anti Cul1 (a.a. 766-776) Rabbit Antibody to Cullin 1 (Cul1), C-terminal (amino acids 766-776)

Specificity: Reacts with human Cullin 1.

Application Details

Application Notes: Suitable for use in ELISA (1:2,000 ? 1:10,000) and Western blot (1:500 ? 1:1,000). The antibody immunoprecipitates in vitro translated protein and protein from cell lysates (using HeLa or NIH-3T3). NOTE: Do not Immunoprecipitate in the presence of NP-40, but rather use 0.1% SDS. An 89.6 kDa band corresponding to human Cul1 is detected. Each laboratory should determine an optimum working titer for use in its particular application. Other applications have not been tested but use in such assays should not necessarily be excluded.

Concentration: Total Protein: 85mg/ml (Refractometry)

Purification: Neat antiserum delipidation and defibrination Product is sterile filtered.

Buffer: Not applicable. Preservative: 0.01% (w/v) Sodium Azide

Storage: Upon receipt, aliquot and store at -20°C or below. Avoid multiple freeze/thaw cycles. Prepare working dilution only prior to immediate use. : This product contains sodium azide, which has been classified as Xn (Harmful) in European Directive 67/548/EEC in the concentration range of 0.1-1.0%. When disposing of this reagent through lead or copper plumbing, flush with copious volumes of water to prevent azide build-up in drains.

Restrictions: For Research Use only

Publications

Kipreos, Lander, Wing et al.: "cul-1 is required for cell cycle exit in C. elegans and identifies a novel gene family." in: Cell, Vol. 85, Issue 6, pp. 829-39, 1996 (PubMed).

Mathias, Johnson, Winey et al.: "Cdc53p acts in concert with Cdc4p and Cdc34p to control the G1-to-S-phase transition and identifies a conserved family of proteins." in: Molecular and cellular biology, Vol. 16, Issue 12, pp. 6634-43, 1997 (PubMed).

Le Meignen, Mossler, Mates et al.: "[Management of immune thrombocytopenic purpura: A prospective study of 147 children from the regional network RH" in: Archives de pediatrie : organe officiel de la Societe francaise de pediatrie, 2008 (PubMed).