Cullin 3 (CUL3) (N-Term AA 1-12) antibody

Antigen: Cullin 3 (CUL3)

Synonyms: CUL-3, Cullin-3, cul3, cul3a, CUL-3-A, MGC79108, Cullin-3-A, cul3b, CUL-3-B, MGC80402, Cullin-3-B, AI467304, AW146203, mKIAA0617, MGC189292, wu:fc37e01, CUL3, ARABIDOPSIS THALIANA CULLIN 3, ARABIDOPSIS THALIANA CULLIN 3A, ATCUL3, ATCUL3A, CUL3A, CULLIN 3, CULLIN 3A, T24P13.25

Binding Site: N-Term AA 1-12

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Conjugate: Un-conjugated

Application: ELISA, Immunoprecipitation (IP), Western Blotting (WB)

Catalog no.: ABIN237095

Additional Information

Immunogen: A synthetic peptide corresponding to amino acids 1-12 of Human Cul3 (N-terminus) coupled to KLH Sequence: MSNLSKGTGSRK

Format: Liquid

Description: Rabbit anti Cul3 (a.a. 1-12) Rabbit Antibody to Cullin 3 (Cul3), N-terminal (amino acids 1-12)

Specificity: Reacts with human Cullin 3.

Synonyms: CUL-3, Cullin-3, cul3, cul3a, CUL-3-A, MGC79108, Cullin-3-A, cul3b, CUL-3-B, MGC80402, Cullin-3-B, AI467304, AW146203, mKIAA0617, MGC189292, wu:fc37e01, CUL3, ARABIDOPSIS THALIANA CULLIN 3, ARABIDOPSIS THALIANA CULLIN 3A, ATCUL3, ATCUL3A, CUL3A, CULLIN 3, CULLIN 3A, T24P13.25

Application Details

Application Notes: Suitable for use in ELISA (1:2,000 ? 1:10,000) and Western blot (1:500 ? 1:1,000). The antibody immunoprecipitates in vitro translated protein and protein from cell lysates (using HeLa or NIH-3T3). An 88.9 kDa band corresponding to human Cul3 is detected. Each laboratory should determine an optimum working titer for use in its particular application. Other applications have not been tested but use in such assays should not necessarily be excluded.

Concentration: Total Protein: 85mg/ml (Refractometry)

Purification: Neat antiserum delipidation and defibrination Product is sterile filtered.

Buffer: Not applicable. Preservative: 0.01% (w/v) Sodium Azide

Storage: Upon receipt, aliquot and store at -20°C or below. Avoid multiple freeze/thaw cycles. Prepare working dilution only prior to immediate use. : This product contains sodium azide, which has been classified as Xn (Harmful), in European Directive 67/548/EEC in the concentration range of 0.1-1.0%. When disposing of this reagent through lead or copper plumbing, flush with copious volumes of water to prevent azide build-up in drains.

Restrictions: For Research Use only

Publications

Kipreos, Lander, Wing et al.: "cul-1 is required for cell cycle exit in C. elegans and identifies a novel gene family." in: Cell, Vol. 85, Issue 6, pp. 829-39, 1996 (PubMed).

Mathias, Johnson, Winey et al.: "Cdc53p acts in concert with Cdc4p and Cdc34p to control the G1-to-S-phase transition and identifies a conserved family of proteins." in: Molecular and cellular biology, Vol. 16, Issue 12, pp. 6634-43, 1997 (PubMed).

Furukawa, He, Borchers et al.: "Targeting of protein ubiquitination by BTB-Cullin 3-Roc1 ubiquitin ligases." in: Nature cell biology, Vol. 5, Issue 11, pp. 1001-7, 2003 (PubMed).

Le Meignen, Mossler, Mates et al.: "[Management of immune thrombocytopenic purpura: A prospective study of 147 children from the regional network RH" in: Archives de pediatrie : organe officiel de la Societe francaise de pediatrie, 2008 (PubMed).