anti-Cullin 5 antibody (CUL5) (N-Term)

Details for Product anti-CUL5 Antibody No. ABIN237097
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N-Term, AA 4-18
(11), (9), (7), (2), (2), (2), (2), (1), (1), (1), (1)
(68), (39), (35), (3), (2), (1), (1), (1), (1)
(67), (1)
This Cullin 5 antibody is un-conjugated
(2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2)
ELISA, Immunoprecipitation (IP), Western Blotting (WB)
(37), (22), (20), (17), (15), (8), (7), (3), (3), (2), (1)
Pubmed 5 references available
Quantity 100 μL
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Availability Please contact our customer support.
Immunogen A synthetic peptide corresponding to AA 4-18 of Human Cul5 (N-terminus) coupled to KLH. Sequence: SNLLKNKGSLQFEDK
Specificity Reacts with human Cullin 5. Cross reactivity with other human cullins may occur.
Characteristics Rabbit anti Cul5 (AA 4-18), Rabbit antibody to Cullin 5 (Cul5), N-terminal (AA 4-18)
Purification Neat antiserum delipidation and defibrination.
Sterility Sterile filtered
Application Notes Suitable for use in ELISA (1:2.000 - 1:10.000) and Western blot (1:500 - 1:1.000). The antibody immunoprecipitates in vitro translated protein and protein from cell lysates (using 293T, U2OS and others). This antibody also co-immunoprecipitates associated proteins. A 90.9 kDa band corresponding to human Cul5 is detected. Each laboratory should determine an optimum working titer for use in its particular application. Other applications have not been tested but use in such assays should not necessarily be excluded.
Restrictions For Research Use only
Format Liquid
Buffer 0.01 % (w/v) Sodium Azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid multiple freeze/thaw cycles. Prepare working dilution only prior to immediate use.
Centrifuge product if not completely clear after standing at room temperature.
Prepare working dilution only prior to immediate use.
Storage -20 °C
Storage Comment Aliquot and store at -20 °C or below.
General Le Meignen, Mossler, Mates et al.: "[Management of immune thrombocytopenic purpura: A prospective study of 147 children from the regional network RH" in: Archives de pediatrie : organe officiel de la Societe francaise de pediatrie, 2008 (PubMed).

Yu, Yu, Liu et al.: "Induction of APOBEC3G ubiquitination and degradation by an HIV-1 Vif-Cul5-SCF complex." in: Science (New York, N.Y.), Vol. 302, Issue 5647, pp. 1056-60, 2003 (PubMed).

Querido, Blanchette, Yan et al.: "Degradation of p53 by adenovirus E4orf6 and E1B55K proteins occurs via a novel mechanism involving a Cullin-containing complex." in: Genes & development, Vol. 15, Issue 23, pp. 3104-17, 2001 (PubMed).

Mathias, Johnson, Winey et al.: "Cdc53p acts in concert with Cdc4p and Cdc34p to control the G1-to-S-phase transition and identifies a conserved family of proteins." in: Molecular and cellular biology, Vol. 16, Issue 12, pp. 6634-43, 1997 (PubMed).

Kipreos, Lander, Wing et al.: "cul-1 is required for cell cycle exit in C. elegans and identifies a novel gene family." in: Cell, Vol. 85, Issue 6, pp. 829-39, 1996 (PubMed).

Catalog No. ABIN237097
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